[Influence and analysis of seven kinds of hemoglobin variants on HbA1c detection]

To explore the effects of seven kinds of hemoglobin variants on two HbA1c detection methods.

Twenty-five hemoglobin variant samples (Hb D, S, Q, G, J, E & F) and 40 control samples were from February 2012 to April 2013 collected. All samples were tested by ion exchange-high performance liquid chromatography system (IE-HPLC) and affinity chromatograghy high performance liquid chromatography (AC-HPLC) respectively.We compared the coincidence between HbA1c results of two instruments and blood glucose and observed the difference between variant and control groups for two methods using statistic software SPSS 19.0.

A high consistency existed between IE-HPLC and AC-HPLC in the control group with no hemoglobin variants (6.68% ± 1.87% vs 6.64% ± 1.99%, P > 0.05) . For the hemoglobin variants group, the results of HbA1c via IE-HPLC were interfered by hemoglobin variants (3.57% ± 3.51% than 4.95% ± 0.57%, P < 0.05). However, HbA1c detection of AC-HPLC had no interference with hemoglobin variants and it demonstrated an excellent correlation with blood glucose.

The results of HbA1c in blood samples containing common hemoglobin variants may be interfered on IE-HPLC to be falsely lower or higher.Only detecting glycated hemoglobin with strong specificity,AC-HPLC is well-correlated with blood glucose and its results are not interfered by common variant hemoglobin.