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T Cell Activation and Clonal Selection

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...

Hybridoma Technology

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation,...

Cross-reactivity

Cross-reactivity

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Updated: Apr 30, 2026

Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells

Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells

Published on: October 22, 2012

T-cell epitope discovery technologies.

Govinda Sharma¹, Robert A Holt²

¹Michael Smith Genome Sciences Centre, BC Cancer Agency, 675 W 10th Avenue, Vancouver, British Columbia V5Z 1L3, Canada; Department of Medical Genetics, University of British Columbia, C201 - 4500 Oak Street, Vancouver, British Columbia V6H 3N1, Canada.

Human Immunology

|April 24, 2014

Summary

This summary is machine-generated.

Discovering T-cell antigens, crucial for medicine and research, has been challenging due to complex cell interactions and vast epitope possibilities. This review explores strategies and technologies to advance T-cell antigen discovery.

Keywords:

Epitopes High-throughput screening Major histocompatibility complex T-cell receptor

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Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

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Published on: March 24, 2017

Area of Science:

Immunology and Molecular Biology
Biomedical Research

Background:

T-cell antigen discovery lags in high-throughput '-omics' due to complex effector T cell-antigen-presenting cell interactions.
The vast T-cell repertoire and numerous potential T-cell epitopes present significant discovery challenges.

Purpose of the Study:

To review the evolution of T-cell antigen discovery strategies.
To examine the technical platforms employed in T-cell antigen discovery.
To assess the current success and limitations in the field.

Main Methods:

Literature review of T-cell antigen discovery strategies.
Analysis of high-throughput '-omics' technologies applied to T-cell research.
Assessment of published data on T-cell epitope identification.

Main Results:

Significant advancements in T-cell antigen discovery methods have been made.
Various technical platforms show promise but face limitations.
The field is progressing towards more comprehensive T-cell epitope characterization.

Conclusions:

Overcoming challenges in T-cell antigen discovery requires innovative strategies and technologies.
Continued development of high-throughput methods is essential for clinical and research applications.
The review highlights the need for integrated approaches to fully exploit T-cell antigen potential.