Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

cGAS/STING sensing in dendritic cells discriminates between daptomycin sensitive and resistant <i>Staphylococcus aureus</i> clinical isolates.

iScience·2026

Same author

Mind the corner: Fillets in cryo-FIB lamella preparation to minimise sample loss caused by stress concentration and lamella breakage.

Journal of structural biology·2025

Same author

AI-directed voxel extraction and volume EM identify intrusions as sites of mitochondrial contact.

The Journal of cell biology·2025

Same author

Mitochondrial damage in muscle specific PolG mutant mice activates the integrated stress response and disrupts the mitochondrial folate cycle.

Nature communications·2025

Same author

High-resolution cryo-EM using a common LaB<sub>6</sub> 120-keV electron microscope equipped with a sub-200-keV direct electron detector.

Science advances·2025

Same author

HMGCS1 variants cause rigid spine syndrome amenable to mevalonic acid treatment in an animal model.

Brain : a journal of neurology·2024

Same journal

Thymidylate synthase inhibitory drugs induce p53-dependent pathways differently.

PloS one·2026

Same journal

Top-down and bottom-up attention for joint pattern classification and reconstruction.

PloS one·2026

Same journal

Short- and long-term scaling behavior of blood pressure and pulse arrival time during sleep in healthy controls and patients with obstructive sleep apnea.

PloS one·2026

Same journal

Double DQN-based secrecy energy efficiency and fairness performance in IRS-assisted NOMA systems with friendly jamming.

PloS one·2026

Same journal

10 recommendations for strengthening citizen science for improved societal and ecological outcomes: A co-produced analysis of challenges and opportunities in the 21st century.

PloS one·2026

Same journal

Paying in public: Peer effects, impression management, and willingness to pay on digital payment platforms.

PloS one·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Apr 30, 2026

Correlative Light and Electron Microscopy CLEM as a Tool to Visualize Microinjected Molecules and their Eukaryotic Sub-cellular Targets

Correlative Light and Electron Microscopy CLEM as a Tool to Visualize Microinjected Molecules and their Eukaryotic Sub-cellular Targets

Published on: May 4, 2012

An improved procedure for subcellular spatial alignment during live-cell CLEM.

Benjamin S Padman¹, Markus Bach¹, Georg Ramm¹

¹Department of Biochemistry and Molecular Biology, Monash University, Clayton campus, Victoria, Australia; Monash Micro Imaging, Monash University, Clayton campus, Victoria, Australia.

|April 24, 2014

Summary

This summary is machine-generated.

This study introduces a simple method using toner particles for live-cell correlative light and electron microscopy (CLEM). This technique simplifies tracking cellular structures for precise ultrastructural analysis.

More Related Videos

Correlative Light Electron Microscopy CLEM for Tracking and Imaging Viral Protein Associated Structures in Cryo-immobilized Cells

Correlative Light Electron Microscopy CLEM for Tracking and Imaging Viral Protein Associated Structures in Cryo-immobilized Cells

Published on: September 7, 2018

Dual-color Correlative Light and Electron Microscopy for the Visualization of Interactions between Mitochondria and Lysosomes

Dual-color Correlative Light and Electron Microscopy for the Visualization of Interactions between Mitochondria and Lysosomes

Published on: September 27, 2024

Related Experiment Videos

Last Updated: Apr 30, 2026

Correlative Light and Electron Microscopy CLEM as a Tool to Visualize Microinjected Molecules and their Eukaryotic Sub-cellular Targets

Correlative Light and Electron Microscopy CLEM as a Tool to Visualize Microinjected Molecules and their Eukaryotic Sub-cellular Targets

Published on: May 4, 2012

Correlative Light Electron Microscopy CLEM for Tracking and Imaging Viral Protein Associated Structures in Cryo-immobilized Cells

Correlative Light Electron Microscopy CLEM for Tracking and Imaging Viral Protein Associated Structures in Cryo-immobilized Cells

Published on: September 7, 2018

Dual-color Correlative Light and Electron Microscopy for the Visualization of Interactions between Mitochondria and Lysosomes

Dual-color Correlative Light and Electron Microscopy for the Visualization of Interactions between Mitochondria and Lysosomes

Published on: September 27, 2024

Area of Science:

Cell Biology
Microscopy Techniques

Background:

Live-cell correlative light and electron microscopy (CLEM) is crucial for studying dynamic cellular processes.
Accurate 3D relocation of cellular regions of interest during sample processing is a major challenge in CLEM.

Purpose of the Study:

To develop a simplified and robust CLEM procedure for precise ultrastructural analysis.
To overcome the technical challenges of relocating specific cellular regions in CLEM.

Main Methods:

A novel CLEM protocol utilizing toner particles from a standard laser printer as orientation markers.
Integration of toner particles with subcellular fluorescence markers (plasma membrane, nucleus) for spatial alignment.
Development of a polymer film holder compatible with inverted microscopes.

Main Results:

Toner particles enable easy, even visual, tracking of regions of interest throughout the CLEM workflow.
Precise subcellular spatial alignment of optical and electron microscopy datasets was achieved.
Successful validation by tracking mitochondria ultrastructure after photo-irradiation.

Conclusions:

The developed toner-based CLEM procedure is inexpensive, robust, and simplifies optical imaging.
This method facilitates accurate ultrastructural analysis of dynamic cellular processes without limiting optical microscope choices.