Related Experiment Video
Updated: Apr 30, 2026

Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis
Published on: May 23, 2021
Functional identification of Proteus mirabilis eptC gene encoding a core lipopolysaccharide phosphoethanolamine
Eleonora Aquilini1, Susana Merino2, Yuriy A Knirel3
1Department Microbiology and Parasitology, Faculty Pharmacy, University of Barcelona, Av. Joan XXIII s/n, 08028 Barcelona, Spain. eaquilini@ub.edu.
Abstract:
By comparison of the Proteus mirabilis HI4320 genome with known lipopolysaccharide (LPS) phosphoethanolamine transferases, three putative candidates (PMI3040, PMI3576, and PMI3104) were identified. One of them, eptC (PMI3104) was able to modify the LPS of two defined non-polar core LPS mutants of Klebsiella pneumoniae that we use as surrogate substrates. Mass spectrometry and nuclear magnetic resonance showed that eptC directs the incorporation of phosphoethanolamine to the O-6 of L-glycero-D-mano-heptose II. The eptC gene is found in all the P. mirabilis strains analyzed in this study. Putative eptC homologues were found for only two additional genera of the Enterobacteriaceae family, Photobacterium and Providencia. The data obtained in this work supports the role of the eptC (PMI3104) product in the transfer of PEtN to the O-6 of L,D-HepII in P. mirabilis strains.
More Related Videos
10:59Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry
Published on: May 21, 2018
08:57Identification of Novel Genes Associated with Alginate Production in Pseudomonas aeruginosa Using Mini-himar1 Mariner Transposon-mediated Mutagenesis
Published on: March 10, 2014
Related Concept Videos
Formation of Lipopolysaccharides
Bacterial Phylum Proteobacteria
Bacterial Gastroenteritis
Bacterial Phylum Tenericutes