Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

Tagging and Fusion Proteins

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

An optimal "Click" formulation strategy for antibody-drug conjugate synthesis.

Bioorganic & medicinal chemistry·2020

Same author

Dissection, Optimization, and Structural Analysis of a Covalent Irreversible DDAH1 Inhibitor.

Biochemistry·2018

Same author

The Taxonomy of Covalent Inhibitors.

Biochemistry·2018

Same author

Selective Covalent Protein Modification by 4-Halopyridines through Catalysis.

Chembiochem : a European journal of chemical biology·2017

Same author

[Effect of ginsenoside total saponinon on regulation of P450 of livers of rats after γ-ray irradiation].

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica·2016

Same author

Lipid-polymer hybrid nanoparticles for the delivery of gemcitabine.

Journal of controlled release : official journal of the Controlled Release Society·2016

Same journal

Bi-modified Ni<sub>3</sub>S<sub>2</sub> promotes selective nitrite-to-hydroxylamine reduction for cyclohexanone oxime synthesis.

Chemical communications (Cambridge, England)·2026

Same journal

Cyclable manganese inventory as a mechanistic descriptor for electrolyte design in rechargeable aqueous Zn-MnO<sub>2</sub> batteries.

Chemical communications (Cambridge, England)·2026

Same journal

Cobalt-iron layered double hydroxides with ligand modification for boosting glycerol electrooxidation-assisted hydrogen production.

Chemical communications (Cambridge, England)·2026

Same journal

Beyond the vacuum: modeling the solid-liquid interface for gas-involving electrocatalysis.

Chemical communications (Cambridge, England)·2026

Same journal

Alkaline-earth carbonate-supported Ru for quinoline hydrogenation: enhanced H<sub>2</sub> activation <i>via</i> electronic metal-support interaction.

Chemical communications (Cambridge, England)·2026

Same journal

Mitigating ionic conductivity limitations <i>in operando</i> electrochemical X-ray photoelectron spectroscopy.

Chemical communications (Cambridge, England)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Apr 30, 2026

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Published on: April 9, 2018

A genetically encoded aldehyde for rapid protein labelling.

Alfred Tuley¹, Yan-Jiun Lee, Bo Wu

¹Department of Chemistry, Texas A&M University, College Station, TX 77843, USA. wliu@chem.tamu.edu.

Chemical Communications (Cambridge, England)

|April 24, 2014

Summary

This summary is machine-generated.

Researchers genetically incorporated 3-formyl-phenylalanine into proteins using a mutant pyrrolysyl-tRNA synthetase-tRNA pair in E. coli. This enables rapid, site-selective protein labeling with hydroxylamine dyes.

More Related Videos

Protease- and Acid-catalyzed Labeling Workflows Employing 18O-enriched Water

Protease- and Acid-catalyzed Labeling Workflows Employing 18O-enriched Water

Published on: February 20, 2013

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling

Published on: September 20, 2016

Related Experiment Videos

Last Updated: Apr 30, 2026

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Published on: April 9, 2018

Protease- and Acid-catalyzed Labeling Workflows Employing 18O-enriched Water

Protease- and Acid-catalyzed Labeling Workflows Employing 18O-enriched Water

Published on: February 20, 2013

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling

Published on: September 20, 2016

Area of Science:

Biochemistry
Molecular Biology
Synthetic Biology

Background:

Site-specific protein modification is crucial for biochemical and biomedical applications.
Genetic incorporation of non-canonical amino acids (ncAAs) offers a powerful tool for protein engineering.
Amber stop codon suppression is a common strategy for introducing ncAAs into proteins.

Purpose of the Study:

To genetically incorporate 3-formyl-phenylalanine (3-FP) into proteins in Escherichia coli.
To demonstrate the utility of 3-FP for site-selective protein labeling.

Main Methods:

Utilized a mutant pyrrolysyl-tRNA synthetase-tRNA(Pyl)(CUA) pair for amber stop codon suppression.
Expressed proteins with site-specifically incorporated 3-FP in E. coli.
Labeled the incorporated 3-FP with hydroxylamine dyes.

Main Results:

Successfully achieved genetic incorporation of 3-FP into proteins at amber mutation sites in E. coli.
Demonstrated that the incorporated 3-FP readily reacts with hydroxylamine dyes.
Achieved rapid and site-selective protein labeling using this method.

Conclusions:

The developed system enables efficient site-specific incorporation of 3-FP into proteins in E. coli.
This method provides a robust platform for rapid and site-selective protein labeling.
This approach has potential applications in chemical biology, drug discovery, and diagnostics.