Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ligand-Gated Ion Channel Receptor: Gating Mechanism01:30

Ligand-Gated Ion Channel Receptor: Gating Mechanism

4.6K
Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
4.6K
Voltage-gated Ion Channels01:26

Voltage-gated Ion Channels

11.2K
Voltage-gated ion channels are transmembrane proteins that open and close in response to changes in the membrane potential. They are present on the membranes of all electrically excitable cells such as neurons, heart, and muscle cells.
Generally, all voltage-gated ion channels have a 'voltage-sensing domain' that spans the lipid bilayer. The charged residues in the sensor move in response to the membrane potential changes that open the channel allowing ions movement. There are several types of...
11.2K
Voltage-gated Ion Channels01:26

Voltage-gated Ion Channels

7.6K
7.6K
The Role of Ion Channels in Neuronal Computation01:19

The Role of Ion Channels in Neuronal Computation

3.2K
A postsynaptic neuron usually receives numerous impulses from several other presynaptic neurons. The axon hillock of the postsynaptic neuron integrates all these signals and determines the likelihood of firing an action potential.
Sometimes a single EPSP is strong enough to induce an action potential in the postsynaptic neuron. However, multiple presynaptic inputs must often create EPSPs around the same time for the postsynaptic neuron to be sufficiently depolarized to fire an action potential....
3.2K
Non-gated Ion Channels01:24

Non-gated Ion Channels

7.3K
Ion channels are specialized proteins on the plasma membrane that allow charged ions to pass down their electrochemical gradient. Their main function is to maintain the membrane potential which is critical for cell viability. These channels are either gated or non-gated and can transport more than a thousand ions within milliseconds for the cellular event to occur.
Compared to the gated ion channels, the non-gated channels, also known as leakage or passive channels, have no gating mechanism....
7.3K
Non-gated Ion Channels01:24

Non-gated Ion Channels

3.7K
3.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

T-type Ca<sup>2+</sup> and persistent Na<sup>+</sup> currents synergistically elevate ventral, not dorsal, entorhinal cortical stellate cell excitability.

Cell reports·2023
Same author

A new HCN1 channelopathy: implications for epilepsy.

Brain : a journal of neurology·2021
Same author

Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels as Drug Targets for Neurological Disorders.

Annual review of pharmacology and toxicology·2020
Same author

The subthreshold-active K<sub>V</sub>7 current regulates neurotransmission by limiting spike-induced Ca<sup>2+</sup> influx in hippocampal mossy fiber synaptic terminals.

Communications biology·2019
Same author

HCN1 channels reduce the rate of exocytosis from a subset of cortical synaptic terminals.

Scientific reports·2017
Same author

Recording Hyperpolarization-Activated Cyclic Nucleotide-Gated Channel Currents (Ih) in Neurons.

Cold Spring Harbor protocols·2016
Same journal

Diving exposure and pulmonary stress.

The Journal of physiology·2026
Same journal

Systems modelling of mitochondrial dynamics in different exercise regimes.

The Journal of physiology·2026
Same journal

Central leptin resistance precedes obesity and drives early endocrine dysfunction.

The Journal of physiology·2026
Same journal

Decoding the molecular memory of obesity using machine learning and microRNA dynamics.

The Journal of physiology·2026
Same journal

Kinematic-calcium loops unravel impaired excitation-contraction coupling in MELAS-affected cardioids.

The Journal of physiology·2026
Same journal

hERG1 channels and potential therapeutics for long QT syndrome.

The Journal of physiology·2026
See all related articles

Related Experiment Video

Updated: Apr 30, 2026

A High-content Assay for Monitoring AMPA Receptor Trafficking
10:34

A High-content Assay for Monitoring AMPA Receptor Trafficking

Published on: January 28, 2019

9.6K

Cortical HCN channels: function, trafficking and plasticity.

Mala M Shah1

  • 1Department of Pharmacology, UCL School of Pharmacy, London, UK mala.shah@ucl.ac.uk.

The Journal of Physiology
|April 24, 2014
PubMed
Summary
This summary is machine-generated.

Hyperpolarization-activated cyclic nucleotide-gated (HCN) channels are found in various neuronal parts in the cortex. These channels influence neuronal excitability by modulating electrical signaling in dendrites and axons.

More Related Videos

Method for Identifying Small Molecule Inhibitors of the Protein-protein Interaction Between HCN1 and TRIP8b
10:20

Method for Identifying Small Molecule Inhibitors of the Protein-protein Interaction Between HCN1 and TRIP8b

Published on: November 11, 2016

7.9K
Recapitulation of an Ion Channel IV Curve Using Frequency Components
10:14

Recapitulation of an Ion Channel IV Curve Using Frequency Components

Published on: February 8, 2011

15.6K

Related Experiment Videos

Last Updated: Apr 30, 2026

A High-content Assay for Monitoring AMPA Receptor Trafficking
10:34

A High-content Assay for Monitoring AMPA Receptor Trafficking

Published on: January 28, 2019

9.6K
Method for Identifying Small Molecule Inhibitors of the Protein-protein Interaction Between HCN1 and TRIP8b
10:20

Method for Identifying Small Molecule Inhibitors of the Protein-protein Interaction Between HCN1 and TRIP8b

Published on: November 11, 2016

7.9K
Recapitulation of an Ion Channel IV Curve Using Frequency Components
10:14

Recapitulation of an Ion Channel IV Curve Using Frequency Components

Published on: February 8, 2011

15.6K

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Cell Biology

Background:

  • Hyperpolarization-activated cyclic nucleotide-gated (HCN) channels are part of the voltage-gated potassium channel superfamily.
  • Unlike typical potassium channels, HCN channels activate at hyperpolarizing potentials and conduct cations.
  • Four HCN subunits exist, with HCN1 and HCN2 predominantly expressed in the cerebral cortex.

Purpose of the Study:

  • To investigate the expression and function of HCN channels in different subcellular compartments of cortical neurons.
  • To understand the role of HCN channels in modulating neuronal excitability and synaptic transmission.

Main Methods:

  • The study likely involved molecular biology techniques to identify HCN subunit expression (e.g., in situ hybridization, Western blotting).
  • Electrophysiological recordings (e.g., patch-clamp) were probably used to assess the functional impact of HCN channels on neuronal excitability.
  • Immunohistochemistry may have been employed to determine the subcellular localization of HCN channels.

Main Results:

  • HCN1 and HCN2 subunits are primarily located in the dendrites of cortical pyramidal neurons, with lower expression in somata and other neuron types.
  • HCN channels are trafficked to dendrites via binding to the chaperone protein TRIP8b.
  • These channels modulate spike firing and synaptic integration by altering membrane resistance and resting membrane potential.
  • HCN channels are also found in cortical axons and synaptic terminals, where they regulate synaptic transmission through varied mechanisms.

Conclusions:

  • HCN channels are expressed across multiple subcellular locations within cortical neurons, including dendrites, somata, axons, and synaptic terminals.
  • The presence of HCN channels in these diverse compartments highlights their complex and varied roles in regulating neuronal function.
  • HCN channels significantly impact neuronal excitability and synaptic transmission in the cortex.