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Related Concept Videos

Thermosensation01:43

Thermosensation

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Peripheral thermosensation is the perception of external temperature. A change in temperature (on the surface of the skin and other tissues) is detected by a family of temperature-sensitive ion channels called Transient Receptor Potential, or TRP, receptors. These receptors are located on free nerve endings. Those detecting cold temperatures are closer to the surface of the skin than the nerve endings detecting warmth. These thermoTRP channels, while temperature selective, have relatively...
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GPCRs Regulate Adenylyl Cylase Activity01:09

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Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...
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Repressible Operon: trp Operon01:21

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The trp operon in Escherichia coli exemplifies a repressible operon. It regulates the synthesis of tryptophan through repressor-mediated transcriptional control and attenuation. This dual regulatory mechanism ensures tryptophan biosynthesis occurs only when needed, conserving cellular resources.Structure of the trp OperonThe trp operon consists of five structural genes (trpE, trpD, trpC, trpB, and trpA) that encode enzymes for tryptophan biosynthesis. These genes are transcribed as a single...
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G Protein-coupled Receptors01:15

G Protein-coupled Receptors

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G Protein-Coupled Receptors or GPCRs are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to sensory stimuli such as light, odors, hormones, cytokines, or neurotransmitters.
GPCRs are also called heptahelical, 7TM, or serpentine receptors, and consist of seven (H1-H7) transmembrane alpha-helices that span the bilayer to form a cylindrical core. The transmembrane helices are connected by three extracellular loops and three...
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G Protein-coupled Receptors01:15

G Protein-coupled Receptors

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GPCR Desensitization01:12

GPCR Desensitization

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G protein-coupled receptor (GPCR) signaling plays a crucial role in cell functioning. GPCR desensitization is an equally essential process. It allows cells to respond to changing environments and regain sensitivity to new stimuli while preventing unnecessary stimulation when no longer needed. Prolonged exposure to stimuli leads to GPCR desensitization. It involves blocking the receptors from binding and activating additional G proteins. This inhibits activation of downstream effectors, thereby...
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Related Experiment Video

Updated: Apr 30, 2026

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy
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Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

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TRPC5.

Alexander V Zholos1

  • 1Department of Biophysics, Educational and Scientific Centre "Institute of Biology", Taras Shevchenko Kiev National University, Kiev, 03022, Ukraine, a.zholos@univ.net.ua.

Handbook of Experimental Pharmacology
|April 24, 2014
PubMed
Summary

Human canonical transient receptor potential channel 5 (TRPC5) is a calcium-permeable channel in the CNS and periphery. TRPC5 plays roles in neurotransmission, electrogenesis, and has implications for neurological disorders.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Physiology

Background:

  • Human canonical transient receptor potential channel 5 (TRPC5) is a Ca(2+)-permeable cation channel.
  • Predominantly expressed in the central nervous system (CNS) and also in peripheral tissues.
  • TRPC5 has been linked to nonsyndromic mental retardation.

Purpose of the Study:

  • To summarize the expression, activation, regulation, and function of TRPC5 channels.
  • To highlight the potential roles of TRPC5 in various physiological and pathological processes.

Main Methods:

  • Literature review and synthesis of existing research on TRPC5.
  • Analysis of TRPC5 expression patterns in different tissues.
  • Examination of TRPC5 activation mechanisms and interacting proteins.

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Purification and Reconstitution of TRPV1 for Spectroscopic Analysis
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Purification and Reconstitution of TRPV1 for Spectroscopic Analysis

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Related Experiment Videos

Last Updated: Apr 30, 2026

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy
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Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

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Purification and Reconstitution of TRPV1 for Spectroscopic Analysis
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Purification and Reconstitution of TRPV1 for Spectroscopic Analysis

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Main Results:

  • TRPC5 is widely expressed in the CNS (hippocampus, cerebellum, amygdala) and periphery (kidney, cardiovascular system).
  • Activated by Gq/Gi pathways, store-dependent mechanisms, and various molecules (NO, lipids, protons, Ca2+).
  • TRPC5 exhibits constitutive activity, responds to stretch and cold, and interacts with ~60 proteins.

Conclusions:

  • TRPC5 channels are versatile regulators of Ca(2+) signaling and electrogenesis.
  • TRPC5's unique properties suggest roles in neurotransmission, axon guidance, vascular function, and neurological conditions like anxiety and seizures.
  • Further research, including knockout studies, is uncovering the specific in vivo functions of TRPC5.