Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Thermosensation01:43

Thermosensation

29.7K
Peripheral thermosensation is the perception of external temperature. A change in temperature (on the surface of the skin and other tissues) is detected by a family of temperature-sensitive ion channels called Transient Receptor Potential, or TRP, receptors. These receptors are located on free nerve endings. Those detecting cold temperatures are closer to the surface of the skin than the nerve endings detecting warmth. These thermoTRP channels, while temperature selective, have relatively...
29.7K
GPCRs Regulate Adenylyl Cylase Activity01:09

GPCRs Regulate Adenylyl Cylase Activity

6.9K
Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...
6.9K
Transducer Mechanism: Enzyme-Linked Receptors01:27

Transducer Mechanism: Enzyme-Linked Receptors

4.4K
Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include:
4.4K
Transducer Mechanism: G Protein–Coupled Receptors01:30

Transducer Mechanism: G Protein–Coupled Receptors

9.0K
G Protein–Coupled Receptors (GPCRs) are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to various stimuli. GPCRs regulate critical physiological pathways and are excellent drug targets for treating diseases such as diabetes, cancer, obesity, depression, or Alzheimer's. Nearly 35% of approved drugs implement their therapeutic effects by selectively interacting with specific GPCRs.
GPCRs are also called heptahelical,...
9.0K
Enzyme-linked Receptors01:00

Enzyme-linked Receptors

64.6K
Enzyme-linked receptors are proteins that act as both receptor and enzyme, activating multiple intracellular signals. This is a large group of receptors that include the receptor tyrosine kinase (RTK) family. Many growth factors and hormones bind to and activate the RTKs.
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
64.6K
G Protein-coupled Receptors01:15

G Protein-coupled Receptors

14.1K
G Protein-Coupled Receptors or GPCRs are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to sensory stimuli such as light, odors, hormones, cytokines, or neurotransmitters.
GPCRs are also called heptahelical, 7TM, or serpentine receptors, and consist of seven (H1-H7) transmembrane alpha-helices that span the bilayer to form a cylindrical core. The transmembrane helices are connected by three extracellular loops and three...
14.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The TRPM7 inhibitor carvacrol suppresses angiogenesis and vasculogenic mimicry in triple-negative breast cancer.

International journal of biological sciences·2026
Same author

Cigarette smoke extract (CSE) reduces expression of functional TRPV4 channels in primary human bronchial epithelial cells differentiated at an air liquid interface (ALI) in vitro.

Archives of toxicology·2026
Same author

ERO1a fosters glioblastoma aggressiveness and metabolic flexibility by regulating mitochondria-associated membrane dynamics.

Nature cell biology·2026
Same author

Open-channel block of human TRPV6 by polyamine spermine.

Nature communications·2026
Same author

Mice lacking β-arrestin-2 in melanocortin 4 receptor-expressing neurons show marked metabolic deficits.

JCI insight·2026
Same author

Platelet Cyclophilin D Drives Cholesterol Crystal Embolism-Related Acute Kidney Injury and Kidney Infarction.

Journal of the American Society of Nephrology : JASN·2026
Same journal

Endothelial Cell Phenotypic Plasticity in Atherosclerosis.

Handbook of experimental pharmacology·2026
Same journal

Endothelial Dysfunction and Neurovascular Alterations in Autism Spectrum Disorder.

Handbook of experimental pharmacology·2026
Same journal

Molecular Mechanisms of Endothelial Shear Stress Mechanotransduction in Health and Disease.

Handbook of experimental pharmacology·2026
Same journal

Microvasculature of the Pancreatic Islets of Langerhans in Health and Diabetes.

Handbook of experimental pharmacology·2026
Same journal

Mechanisms of Actions of Physiological, Pharmacological, and Toxicological Dietary Bioactive Inorganic Boron.

Handbook of experimental pharmacology·2026
Same journal

BNCT Plus Luminescence: New Paradigm for Boron-Containing Drug Design.

Handbook of experimental pharmacology·2026
See all related articles

Related Experiment Video

Updated: Apr 30, 2026

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy
08:27

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

Published on: January 7, 2019

8.6K

TRPC6: physiological function and pathophysiological relevance.

Alexander Dietrich1, Thomas Gudermann

  • 1Walther-Straub-Institute for Pharmacology and Toxicology, School of Medicine, LM-University of Munich, 80336, Munich, Germany, Alexander.Dietrich@lrz.uni-muenchen.de.

Handbook of Experimental Pharmacology
|April 24, 2014
PubMed
Summary
This summary is machine-generated.

Transient Receptor Potential Canonical 6 (TRPC6) channels are crucial for physiological functions like smooth muscle contraction and kidney filtration. TRPC6 blockers show promise for treating diseases involving overactive TRPC6 channels.

More Related Videos

Purification of Endogenous Drosophila Transient Receptor Potential Channels
08:39

Purification of Endogenous Drosophila Transient Receptor Potential Channels

Published on: December 28, 2021

1.8K
Method for Identifying Small Molecule Inhibitors of the Protein-protein Interaction Between HCN1 and TRIP8b
10:20

Method for Identifying Small Molecule Inhibitors of the Protein-protein Interaction Between HCN1 and TRIP8b

Published on: November 11, 2016

7.9K

Related Experiment Videos

Last Updated: Apr 30, 2026

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy
08:27

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

Published on: January 7, 2019

8.6K
Purification of Endogenous Drosophila Transient Receptor Potential Channels
08:39

Purification of Endogenous Drosophila Transient Receptor Potential Channels

Published on: December 28, 2021

1.8K
Method for Identifying Small Molecule Inhibitors of the Protein-protein Interaction Between HCN1 and TRIP8b
10:20

Method for Identifying Small Molecule Inhibitors of the Protein-protein Interaction Between HCN1 and TRIP8b

Published on: November 11, 2016

7.9K

Area of Science:

  • Physiology
  • Molecular Biology
  • Ion Channel Research

Background:

  • TRPC6 is a non-selective cation channel permeable to Ca(2+) and Na(+).
  • It is activated by diacylglycerol (DAG) and regulated by phosphorylation and phosphoinositides.
  • TRPC6 exhibits a doubly rectifying current-voltage relationship.

Purpose of the Study:

  • To elucidate the physiological roles of TRPC6.
  • To investigate TRPC6 involvement in smooth muscle contraction, pulmonary edema, kidney function, and neuronal protection.
  • To explore potential therapeutic applications of TRPC6 blockers.

Main Methods:

  • Analysis of a Trpc6 knockout mouse model.
  • Electrophysiological characterization of TRPC6 channel activity.
  • Investigation of TRPC6 expression patterns and regulatory mechanisms.

Main Results:

  • TRPC6 mediates Na(+) influx driving smooth muscle contraction.
  • TRPC6 regulates pulmonary endothelial cell permeability, impacting lung edema.
  • TRPC6 is vital for kidney podocyte slit diaphragm integrity and neuronal protection post-ischemia.

Conclusions:

  • TRPC6 plays significant roles in smooth muscle, pulmonary endothelium, kidney podocytes, and neurons.
  • Further research is needed to understand TRPC6 functions in immune and blood cells.
  • TRPC6 blockers represent a potential therapeutic strategy for diseases with TRPC6 hyperactivity.