High-throughput sequencing in veterinary infection biology and diagnostics

Area of Science:

• Veterinary Medicine
• Microbiology
• Biotechnology

Background:

• Sanger sequencing paved the way for advanced pathogen detection methods.
• High-throughput sequencing (HTS) technologies have significantly reduced DNA sequencing costs.
• This has increased the accessibility of advanced molecular diagnostic tools for laboratories.

Purpose of the Study:

• To summarize HTS approaches and their diagnostic applications in veterinary medicine.
• To discuss the potential of HTS for pathogen identification and disease control.
• To outline future needs, particularly in bioinformatic resources.

Main Methods:

• Review of existing high-throughput sequencing (HTS) methodologies.
• Discussion of diagnostic applicability of HTS in veterinary contexts.
• Exploration of future directions and resource requirements.

Main Results:

• HTS provides powerful tools for detecting and identifying diverse pathogens like viruses and bacteria.
• Reduced costs associated with HTS make it a viable option for routine diagnostics.
• The technology shows significant promise for improving infectious disease diagnosis and control.

Conclusions:

• HTS is a transformative technology for veterinary diagnostics.
• Further development of bioinformatic resources is crucial for maximizing HTS utility.
• Implementing HTS can enhance the surveillance and management of infectious diseases in animals.