Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Biophysical Diffusion MRI Models Better Identify White Matter Tracts in Edema.

Tomography (Ann Arbor, Mich.)·2026
Same author

Preterm Birth Increases Susceptibility to Hyperglycemia-Induced Kidney Injury With Sex-Specific Differences in Structural and Molecular Responses.

Endocrinology, diabetes & metabolism·2026
Same author

Measuring nephron number in the healthy and diabetic rat kidney in vivo using MRI without contrast agents.

American journal of physiology. Renal physiology·2026
Same author

Using diffusion MRI to relate hippocampal subfield microstructure to delayed verbal memory in cognitively intact individuals at genetic risk for developing Alzheimer's disease.

Experimental gerontology·2026
Same author

Biomarkers.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2025
Same author

Brain Volumetric Changes Post-COVID-19: A Systematic Review.

Brain sciences·2025

Related Experiment Video

Updated: Apr 30, 2026

Stable Aqueous Suspensions of Manganese Ferrite Clusters with Tunable Nanoscale Dimension and Composition
10:45

Stable Aqueous Suspensions of Manganese Ferrite Clusters with Tunable Nanoscale Dimension and Composition

Published on: February 5, 2022

3.9K

Disruptive chemical doping in a ferritin-based iron oxide nanoparticle to decrease r2 and enhance detection with

M Veronica Clavijo Jordan1, Scott C Beeman, Edwin J Baldelomar

  • 1School of Biological and Health Systems Engineering, Arizona State University, Tempe, Arizona, USA.

Contrast Media & Molecular Imaging
|April 26, 2014
PubMed
Summary

Researchers developed novel paramagnetic WFe nanoparticles for molecular MRI. These agents offer low transverse relaxivity, enabling sensitive T1-weighted imaging in vivo for targeted applications.

Keywords:
FerritinMRINanoparticlesTungsten

More Related Videos

Synthesis of Functionalized Magnetic Nanoparticles, Their Conjugation with the Siderophore Feroxamine and its Evaluation for Bacteria Detection
15:03

Synthesis of Functionalized Magnetic Nanoparticles, Their Conjugation with the Siderophore Feroxamine and its Evaluation for Bacteria Detection

Published on: June 16, 2020

9.2K
Synthesis of Cationized Magnetoferritin for Ultra-fast Magnetization of Cells
10:23

Synthesis of Cationized Magnetoferritin for Ultra-fast Magnetization of Cells

Published on: December 13, 2016

11.3K

Related Experiment Videos

Last Updated: Apr 30, 2026

Stable Aqueous Suspensions of Manganese Ferrite Clusters with Tunable Nanoscale Dimension and Composition
10:45

Stable Aqueous Suspensions of Manganese Ferrite Clusters with Tunable Nanoscale Dimension and Composition

Published on: February 5, 2022

3.9K
Synthesis of Functionalized Magnetic Nanoparticles, Their Conjugation with the Siderophore Feroxamine and its Evaluation for Bacteria Detection
15:03

Synthesis of Functionalized Magnetic Nanoparticles, Their Conjugation with the Siderophore Feroxamine and its Evaluation for Bacteria Detection

Published on: June 16, 2020

9.2K
Synthesis of Cationized Magnetoferritin for Ultra-fast Magnetization of Cells
10:23

Synthesis of Cationized Magnetoferritin for Ultra-fast Magnetization of Cells

Published on: December 13, 2016

11.3K

Area of Science:

  • Biomedical Engineering
  • Materials Science
  • Nanotechnology

Background:

  • Superparamagnetic iron oxide nanoparticles (SPIONs) are common MRI contrast agents but often have high transverse relaxivity (r2), limiting their use in T1-weighted molecular imaging.
  • Existing T1-shortening agents have limitations, including low metal payload or T2 shortening effects that restrict detectable concentration ranges.

Purpose of the Study:

  • To create flexible, paramagnetic nanoparticle contrast agents for in vivo molecular MRI with low r2.
  • To develop a novel contrast agent suitable for sensitive T1-weighted molecular imaging.

Main Methods:

  • Inorganic doping of iron oxide crystals with tungsten and iron within the apoferritin cavity to form WFe nanoparticles.
  • Characterization of nanoparticle magnetic properties, including coercivity and saturation magnetization.
  • Measurement of longitudinal (r1) and transverse (r2) relaxivity.
  • In vivo T1-weighted MRI detection of WFe nanoparticles in rat brains and kidneys.
  • In vitro cytotoxicity assessment.

Main Results:

  • WFe nanoparticles exhibited paramagnetic properties with localized atomic magnetic moments, unlike native ferritin.
  • Achieved significantly increased longitudinal relaxivity (r1 = 4870 mm⁻¹s⁻¹) and reduced transverse relaxivity (r2 = 9076 mm⁻¹s⁻¹).
  • Demonstrated in vivo detection of WFe nanoparticles at concentrations from 20 to 400 nm using T1-weighted MRI.
  • Confirmed non-cytotoxicity up to 700 nm concentrations.

Conclusions:

  • The developed WFe apoferritin nanoparticles are effective paramagnetic contrast agents for in vivo molecular MRI.
  • Their low r2 and high r1 enable sensitive T1-weighted imaging, overcoming limitations of traditional SPIONs.
  • These non-cytotoxic nanoparticles show potential for targeted molecular MRI applications.