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Differences in lectin binding patterns of normal human endometrium between proliferative and secretory phases
1Department of Obstetrics and Gynecology, School of Medicine, Keio University, Tokyo, Japan.
Histochemistry
|January 1, 1989
Summary
Lectin binding patterns in human endometrium change between proliferative and secretory phases. DBA and SBA lectins show significant increases in binding, indicating distinct cellular changes during the menstrual cycle.
Area of Science:
- Histology
- Cell Biology
- Gynecology
Background:
- The endometrium undergoes cyclical changes.
- Lectin binding reflects cell surface glycoconjugates.
- Understanding endometrial lectin patterns is crucial for reproductive health.
Purpose of the Study:
- To investigate lectin binding patterns in normal human endometrium during proliferative and secretory phases.
- To identify lectins that exhibit significant changes in binding between the two phases.
Main Methods:
- Light and electron microscopy were used.
- Seven lectins (ConA, WGA, RCA, PNA, UEA-1, DBA, SBA) were employed.
- Avidin-biotin-peroxidase complex (ABC) technique was utilized.
Main Results:
- ConA, WGA, RCA stained glandular cells in both phases with minimal change.
- UEA-1 showed reduced binding in the secretory phase.
- DBA and SBA exhibited significantly increased binding in the secretory phase, particularly in the Golgi apparatus.
Conclusions:
- Lectin binding patterns, especially DBA and SBA, differ significantly between proliferative and secretory endometrium.
- These changes suggest alterations in endometrial cell surface glycosylation during the menstrual cycle.
- DBA and SBA may serve as markers for endometrial phase identification.