Related Experiment Videos
A novel in situ colony screening method to detect human immunodeficiency virus reverse transcriptase activity
1Department of Biochemistry, Columbia University College of Physicians and Surgeons, New York, New York 10032.
The Journal of Biological Chemistry
|October 5, 1989
Abstract:
An in situ screening procedure is described that permits the detection of RNA-dependent DNA polymerase activity in bacterial colonies expressing the reverse transcriptase of the human immunodeficiency virus. The procedure has been applied to the isolation of rare pseudorevertants of an inactive parental mutant.