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Updated: Apr 30, 2026

Bacterial Immobilization for Imaging by Atomic Force Microscopy
Published on: August 10, 2011
Comparison and evaluation of immobilization methods for preparing bacterial probes using acidophilic bioleaching
Mengxue Diao1, Elena Taran2, Stephen M Mahler3
1School of Chemical Engineering, The University of Queensland, St Lucia, QLD 4072, Australia.
Abstract:
We evaluated different strategies for constructing bacterial probes for atomic force microscopy studies of bioleaching Acidithiobacillus thiooxidans interacting with pyrite mineral surfaces. Of three available techniques, the bacterial colloidal probe technique is the most reliable and provides a versatile platform for quantifying true interactive forces between bioleaching microorganisms and mineral surfaces.
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