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Methods for quantifying Staphylococcus aureus in indoor air.

C-W Chang1, L-J Wang

  • 1Institute of Environmental Health, College of Public Health, National Taiwan University, Taipei, Taiwan, Republic of China; Center for Research on Environmental and Occupational Health, National Taiwan University, Taipei, Taiwan; Research Center for Genes, Environmental and Human Health, National Taiwan University, Taipei, Taiwan.

Indoor Air
|April 30, 2014
PubMed
Summary
This summary is machine-generated.

Efficiently sampling indoor Staphylococcus aureus (S. aureus) requires optimal methods. The BioSampler with Tween mixture (TM) and 4°C storage best preserves bacterial viability for accurate exposure risk assessment.

Keywords:
Air samplingAndersenBioSamplerSample storageStaphylococcus aureusTween mixture

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Area of Science:

  • Environmental microbiology
  • Air sampling technology
  • Bacterial exposure assessment

Background:

  • Staphylococcus aureus is detected in indoor air and linked to human infections.
  • Accurate quantification of S. aureus is crucial for characterizing human exposure risks.
  • Efficient sampling and storage methods are needed for reliable indoor air quality monitoring.

Purpose of the Study:

  • To evaluate the impact of different sampler types, collection fluids, and sampling times on S. aureus recovery.
  • To assess the effect of storage conditions on bacterial concentration over time.
  • To determine the optimal method for sampling and storing S. aureus from indoor air.

Main Methods:

  • Laboratory study comparing three samplers: all-glass impinger (AGI-30), BioSampler, and Andersen one-stage sampler (Andersen 1-STG).
  • Evaluation of three collection fluids: deionized water (DW), phosphate-buffered saline (PBS), and Tween mixture (TM).
  • Assessment of sampling times (3-60 min) and storage conditions (4°C and 23°C for 48 h) on bacterial recovery and concentration.

Main Results:

  • The BioSampler demonstrated superior performance compared to Andersen 1-STG and AGI-30 (P < 0.05).
  • Tween mixture (TM) was more effective than PBS and DW (P < 0.05) for cell recovery.
  • Extended sampling times negatively impacted cell recovery in PBS, while TM showed increased recovery at 6-15 min. PBS samples degraded during storage at 4°C and 23°C, whereas TM samples remained stable at 4°C.

Conclusions:

  • The BioSampler combined with Tween mixture (TM) is the recommended method for sampling S. aureus in indoor air.
  • Storage at 4°C is optimal for maintaining bacterial concentrations in collected samples.
  • These findings provide a basis for improved indoor air quality monitoring and human exposure risk assessment for S. aureus.