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Updated: Apr 30, 2026

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Specific Labeling of Mitochondrial Nucleoids for Time-lapse Structured Illumination Microscopy
Published on: June 4, 2020
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Hoechst tagging: a modular strategy to design synthetic fluorescent probes for live-cell nucleus imaging
Akinobu Nakamura1, Kazumasa Takigawa, Yasutaka Kurishita
1Department of Bioengineering, Nagaoka University of Technology, 1603-1 Kamitomioka, Nagaoka, Niigata 940-2188, Japan. tsukiji@vos.nagaokaut.ac.jp.
Abstract:
We report a general strategy to create small-molecule fluorescent probes for the nucleus in living cells. Our strategy is based on the attachment of the DNA-binding Hoechst compound to a fluorophore of interest. Using this approach, simple fluorescein, BODIPY, and rhodamine dyes were readily converted to novel turn-on fluorescent nucleus-imaging probes.

