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Updated: Apr 30, 2026
Biolistic Transformation of a Fluorescent Tagged Gene into the Opportunistic Fungal Pathogen Cryptococcus neoformans
Published on: March 19, 2015
Development and validation of benomyl birdseed agar for the isolation of Cryptococcus neoformans and Cryptococcus
Cau D Pham1, Stacey Ahn, Lance A Turner
1Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA, USA.
Abstract:
One of the difficulties of isolating Cryptococcus neoformans and Cryptococcus gattii from environmental samples is the abundant overgrowth of other yeast and mold species that occurs on the plates. Here we report the application of benomyl to Guizotia abyssinica seed extract growth medium to improve the isolation of C. neoformans and C. gattii from environmental samples. We validated this medium by recovering C. neoformans and C. gattii from convenience soils and swabs from a region of the United States where these yeasts are endemic.
