Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

12.9K
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
12.9K
The DNA Helix01:16

The DNA Helix

129.4K
Overview
129.4K
The DNA Helix01:07

The DNA Helix

19.9K
Deoxyribonucleic acid, or DNA, is the genetic material responsible for passing traits from generation to generation in all organisms and most viruses. DNA is composed of two strands of nucleotides that wind around each other to form a spring-like structure called a double helix. However, the double helix is not perfectly symmetrical. Instead, there are regularly occurring grooves in the structure. The major groove occurs where the sugar-phosphate backbones are relatively far apart. This space...
19.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Quantitative proteomics identifies surfactant-resistant alpha-synuclein in cerebral cortex of Parkinsonism-dementia complex of Guam but not Alzheimer's disease or progressive supranuclear palsy.

The American journal of pathology·2007
Same author

Different supramolecular assemblies in two 1:1 proton-transfer compounds of sulfobenzoic acids with aromatic amines.

Acta crystallographica. Section C, Crystal structure communications·2007
Same author

Identification of proteins involved in microglial endocytosis of alpha-synuclein.

Journal of proteome research·2007
Same author

Biomarkers for Alzheimer's disease.

Expert review of neurotherapeutics·2007
Same author

[Role of sympathetic nerve activity and arterial endothelial function in pathogenesis of hypertension in patients with obstructive sleep apnea-hypopnea syndrome].

Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases·2007
Same author

Subsequently enhanced CPP to morphine following chronic but not acute footshock stress associated with corticosterone mechanism in rats.

The International journal of neuroscience·2007

Related Experiment Video

Updated: Apr 30, 2026

DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers
08:00

DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers

Published on: October 25, 2017

6.6K

[A fluoride-sensor for kink structure in DNA condensation process].

Yan-Hui Liu, Jing Zhang, Ying-Bing Chen

    Guang Pu Xue Yu Guang Pu Fen Xi = Guang Pu
    |May 3, 2014
    PubMed
    Summary
    This summary is machine-generated.

    Fluoride ions induce DNA kinks during condensation, unlike chlorine ions. This discovery enabled the development of a fluoride-sensor for detecting DNA kink structures, specifically in spermine-DNA condensation.

    More Related Videos

    Studying DNA Looping by Single-Molecule FRET
    11:27

    Studying DNA Looping by Single-Molecule FRET

    Published on: June 28, 2014

    14.8K
    Analyzing and Building Nucleic Acid Structures with 3DNA
    16:24

    Analyzing and Building Nucleic Acid Structures with 3DNA

    Published on: April 26, 2013

    20.3K

    Related Experiment Videos

    Last Updated: Apr 30, 2026

    DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers
    08:00

    DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers

    Published on: October 25, 2017

    6.6K
    Studying DNA Looping by Single-Molecule FRET
    11:27

    Studying DNA Looping by Single-Molecule FRET

    Published on: June 28, 2014

    14.8K
    Analyzing and Building Nucleic Acid Structures with 3DNA
    16:24

    Analyzing and Building Nucleic Acid Structures with 3DNA

    Published on: April 26, 2013

    20.3K

    Area of Science:

    • Molecular Biology
    • Biophysical Chemistry
    • Genetics

    Background:

    • The 'kink' structure in DNA condensation, proposed by Bloomfield, lacked experimental validation.
    • Understanding DNA condensation is crucial for processes like viral packaging and gene regulation.

    Purpose of the Study:

    • To experimentally investigate the effects of halogens (fluoride and chlorine) on polyamine-DNA condensation.
    • To develop a sensor for detecting DNA 'kink' structures based on observed halogen effects.
    • To differentiate between DNA condensation mechanisms involving spermine and spermidine.

    Main Methods:

    • Utilized UV Spectrophotometry to monitor changes in the polyamine-DNA condensation system.
    • Introduced fluoride and chlorine ions to observe their distinct interactions with DNA and polyamines.

    Main Results:

    • Fluoride ions induced a significant blue-shift and hyperchromicity in the spermine-DNA system, indicating 'kink' formation.
    • Chlorine ions caused only minor hyperchromicity in both spermine-DNA and spermidine-DNA systems.
    • A novel fluoride-sensor was developed, specifically detecting the second type of 'kink' structure in spermine-DNA condensation.

    Conclusions:

    • Fluoride ions play a unique role in inducing DNA 'kink' structures during spermine-mediated condensation.
    • The experimental evidence supports the existence of DNA 'kink' structures, validating Bloomfield's hypothesis.
    • The developed fluoride-sensor offers a new tool for studying DNA structural dynamics.