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Updated: Apr 30, 2026

Determination of the Relative Potency of an Anti-TNF Monoclonal Antibody mAb by Neutralizing TNF Using an In Vitro Bioanalytical Method
Published on: September 16, 2017
β-actin in the signaling of transmembrane TNF-α-mediated cytotoxicity
Hui Chen1, Yan Leng, Zhuoya Li
1Department of Immunology, Medical College, Wuhan University of Science and Technology, Wuhan, Hubei, PR China.
Abstract:
To study the role of β-actin in the signaling of transmembrane TNF-α (tmTNF-α)-mediated cytotoxicity, we mainly used bioassay and apoptosis assay, detection of the visual changes in β-actin and intracellular translocation of signal molecules in response to tmTNF-α, and analysis of the signal molecules coupled or uncoupled with TNFR2 complex. These protocols might also be used to investigate the signaling events mediated by other transmembrane cytokines or members of TNF superfamily, which have transmembrane and soluble forms, as well as the involvement of actin cytoskeleton in signal transduction pathways.
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