Related Experiment Video
Updated: Apr 30, 2026
07:51
Preparation of Primary Myogenic Precursor Cell/Myoblast Cultures from Basal Vertebrate Lineages
Published on: April 30, 2014
20.0K
Stac3 inhibits myoblast differentiation into myotubes
Xiaomei Ge1, Yafei Zhang1, Sungwon Park1
1Department of Animal and Poultry Sciences, Virginia Tech, Blacksburg, Virginia, United States of America.
Plos One
|May 3, 2014
Summary
The Stac3 gene inhibits skeletal muscle cell differentiation. Research shows Stac3 knockdown or knockout accelerates myoblast differentiation, while Stac3 overexpression slows it, suggesting Stac3 prevents premature muscle development.
Area of Science:
- Molecular Biology
- Developmental Biology
- Muscle Physiology
Background:
- Stac3 (SH3 and cysteine-rich domain-containing protein 3) is a skeletal muscle-specific protein.
- Its precise function in skeletal muscle development and contraction remains largely undefined.
- Myoblast proliferation and differentiation are critical processes in muscle development.
Purpose of the Study:
- To investigate the role of Stac3 in myoblast proliferation and differentiation.
- To elucidate the regulatory mechanism of Stac3 in muscle development.
Main Methods:
- Utilized C2C12 myoblast cell line for in vitro studies.
- Employed siRNA-mediated knockdown and plasmid-mediated overexpression of Stac3.
- Generated Stac3 knockout mouse embryos for comparative analysis.
- Assessed myoblast proliferation, differentiation, fusion index, and expression of myogenic markers (myogenin, myosin heavy chain).
Main Results:
- Stac3 manipulation (knockdown or overexpression) did not affect C2C12 myoblast proliferation.
- Stac3 knockdown promoted myoblast differentiation, indicated by increased fusion index and myogenic marker expression.
- Stac3 overexpression inhibited myoblast differentiation.
- Stac3 knockout myoblasts exhibited accelerated differentiation compared to wild-type.
Conclusions:
- Endogenous Stac3 plays an inhibitory role in myoblast differentiation.
- Stac3 may function to prevent precocious myoblast differentiation during skeletal muscle development.
- Dysfunctional myofibers can result from premature differentiation, highlighting Stac3's importance in regulating this process.
More Related Videos
Related Concept Videos
01:21Satellite Stem Cells and Muscular Dystrophy
1.7K
Satellite stem cells or myosatellite cells are quiescent stem cells that Alexander Mauro first identified in 1961. These cells are located between the sarcolemma, the plasma membrane of muscle fibers, and the basal lamina, the connective tissue sheath covering it. These mononucleated cells are activated in response to muscle injury, can transform into myoblasts, and may form or repair muscle fibers. Myosatellite cells can provide additional myonuclei for muscle regeneration or return to a...
1.7K
01:13Formation of Muscle Fibers from Myoblasts
5.8K
De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
5.8K
01:15Drugs that Stabilize Microtubules
2.2K
Microtubules are dynamic structures that undergo cycles of catastrophe and rescue. The microtubules play a central role in cell division by forming the spindle apparatus for segregating the chromosomes. This makes them ideal targets for regulating dividing cells in tumors and malignant cancer cells. Microtubule stabilizing drugs help stabilize the microtubule formation and promote its polymerization. Paclitaxel was the first microtubule stabilizing agent used as anticancer drug in chemotherapy...
2.2K
01:45Destabilization of Microtubules
2.9K
The destabilization of microtubules can occur during different stages of the microtubule lifecycle, such as nucleation or elongation. It can take place at either end of the microtubule or in the microtubule lattices as a whole. The lifespan of individual microtubules within a cell varies according to the cell type and stage of the cell cycle. During interphase, the lifespan of the microtubule is about 30 minutes, while during cell division, it is about 15 minutes. In axonal microtubules of...
2.9K
02:23Master Transcription Regulators
6.1K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.1K
01:26Directly Acting Muscle Relaxants: Dantrolene and Botulinum Toxin
2.2K
Directly acting muscle relaxants like dantrolene and botulinum toxin (BoNT) have distinct mechanisms and applications. Dantrolene, a hydantoin derivative, acts on the ryanodine receptor (RYR1) in skeletal muscle cells. RYR1 are calcium channels present at the sarcoplasmic reticulum membrane. In response to excitation, they release calcium ions from the sarcoplasmic reticulum to the cytosol. Calcium promotes actin-myosin-mediated contraction of muscles.
The binding of dantrolene to the RYR1...
The binding of dantrolene to the RYR1...
2.2K