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Updated: Apr 30, 2026

A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting
Published on: March 9, 2017
Flow injection analysis of thiamazole based on strong Ru(bpy)3 (2+) co-reactant electrochemiluminescence
Dexian Kong1, Qinglu Li, Jiang Jiang
1School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China; MOE Key Laboratory of Analysis and Detection Technology for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, and Department of Chemistry, Fuzhou University, Fujian, 350108, China.
Abstract:
Based on the strong electrochemiluminescence (ECL) reaction between thiamazole and tris(2,2'-bipyridine)ruthenium(II) (Ru(bpy)3 (2+) ), a sensitive, simple and rapid flow injection analysis method for the determination of thiamazole was developed. When a Pt working electrode was maintained at a potential of +1.50 V (vs Ag/AgCl) in pH 12.0 H3 PO4 -NaOH solution containing thiamazole and Ru(bpy)3 (2+) at a flow rate of 1.0 mL/min, a linear range of 2.0 × 10(-7) -1.0 × 10(-4) mol/L with a detection limit of 5.0 × 10(-8) mol/L was obtained for the detection of thiamazole. The method showed good reproducibility with a relative standard deviation (RSD) of 0.75%. The method has been successfully applied to the determination of thiamazole in spiked animal feeds. In addition, a co-reactant ECL mechanism was proposed for the thiamazole-Ru(bpy)3 (2+) system.
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