Related Experiment Video

Updated: Apr 30, 2026

Murine Aortic Crush Injury: An Efficient In Vivo Model of Smooth Muscle Cell Proliferation and Endothelial Function
06:14

Murine Aortic Crush Injury: An Efficient In Vivo Model of Smooth Muscle Cell Proliferation and Endothelial Function

Published on: June 11, 2017

7.8K

Platelet-derived growth factor-BB induces matrix metalloproteinase-2 expression and rat vascular smooth muscle cell

Ying Cui1, Yin-Wei Sun, Hai-Shuang Lin

  • 1Department of Biochemistry and Molecular Biology, Dalian Medical University, Lvshun, Dalian, 116044, People's Republic of China.

Molecular and Cellular Biochemistry
|May 6, 2014
PubMed

Insights

Platelet-derived growth factor-BB (PDGF-BB) increases matrix metalloproteinase-2 (MMP-2) in vascular cells. This process involves Rho-associated protein kinase (ROCK) and specific kinases, offering insights into atherosclerosis mechanisms.

Area of Science:

  • Cardiovascular Biology
  • Molecular Cell Biology
  • Atherosclerosis Research

Background:

  • Matrix metalloproteinases (MMPs) are crucial in cardiovascular disease pathogenesis.
  • MMP expression is modulated by growth factors, inflammatory markers, and cytokines.

Purpose of the Study:

  • To investigate the role of platelet-derived growth factor-BB (PDGF-BB) in regulating MMP-2 expression in vascular smooth muscle cells (VSMC).
  • To elucidate the signaling pathways, including Rho-associated protein kinase (ROCK) and mitogen-activated protein kinases (MAPKs), involved in PDGF-BB-induced MMP-2 expression.

Main Methods:

  • VSMC were treated with PDGF-BB to assess MMP-2 expression.
  • Inhibitors of Rho-associated protein kinase (ROCK) (Y-27632) and MAPKs (U0126, SB203580) were used.
  • Small interfering RNA (siRNA) technology was employed to suppress ROCK-1/2 expression.

Main Results:

  • PDGF-BB significantly increased MMP-2 expression in a dose- and time-dependent manner.
  • Inhibition or suppression of ROCK markedly reduced PDGF-BB-induced MMP-2 expression.
  • Inhibition of extracellular signal-regulated kinase (ERK) and p38 MAPK also attenuated MMP-2 induction, indicating their involvement.

Conclusions:

  • PDGF-BB signaling in VSMC induces MMP-2 expression through a pathway involving ERK and p38 MAPK phosphorylation.
  • ROCK plays a significant regulatory role in PDGF-BB-mediated MMP-2 induction.
  • These findings reveal a novel mechanism contributing to atherosclerosis development.

More Related Videos

Calcification of Vascular Smooth Muscle Cells and Imaging of Aortic Calcification and Inflammation08:43

Calcification of Vascular Smooth Muscle Cells and Imaging of Aortic Calcification and Inflammation

Published on: May 31, 2016

19.7K
Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling07:49

Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling

Published on: August 3, 2018

10.1K

Related Concept Videos

MAPK Signaling Cascades01:07

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
7.3K
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.9K
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.2K
Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
6.3K
Small GTPases - Ras and Rho01:24

Small GTPases - Ras and Rho

Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
4.4K
Role of Matrix Metalloproteases in Degradation of ECM01:23

Role of Matrix Metalloproteases in Degradation of ECM

Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult...
2.9K