Confirmation of isolates of Listeria by conventional and real-time PCR
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Detection of Foodborne Bacterial Pathogens from Individual Filth Flies
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David Rodríguez-Lázaro1, Marta Hernández
1Instituto Tecnológico Agrario (ITACyL), Junta de Castilla y León, Ctra. Burgos, km119, 47071, Valladolid, Spain, rodlazda@gmail.com.
Methods in Molecular Biology (Clifton, N.J.)
|May 6, 2014
Abstract:
Polymerase chain reaction (PCR) is an invaluable diagnostic technique in microbiology for rapid and specific detection and confirmation of microbial isolates from food and the environment. PCR is a simple, sensitive, specific, and reproducible assay and can be performed in conventional or in real-time formats. Here, we describe the application of real-time and conventional PCR-based methods for confirmation of presumptive Listeria isolates.

