Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Lagging Strand Synthesis01:59

Lagging Strand Synthesis

38.1K
During replication, the complementary strands in double-stranded DNA are synthesized at different rates. Replication first begins on the leading strand. Replication starts later, occurs more slowly, and proceeds discontinuously on the lagging strand.
There are several major differences between synthesis of the leading strand and synthesis of the lagging strand. 1) Leading strand synthesis happens in the direction of replication fork opening, whereas lagging strand synthesis happens in the...
38.1K
DNA as a Genetic Template02:05

DNA as a Genetic Template

22.2K
Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
22.2K
The Replisome03:01

The Replisome

31.2K
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
31.2K
DNA Topoisomerases02:02

DNA Topoisomerases

32.3K
Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
Types and Mechanism of action
Topoisomerases are divided into two main types. ...
32.3K
DNA Helicases00:55

DNA Helicases

19.4K
DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
19.4K
DNA Replication02:40

DNA Replication

54.3K
DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication...
54.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bisdemethoxycurcumin attenuates cisplatin-induced renal injury through anti-apoptosis, anti-oxidant and anti-inflammatory.

European journal of pharmacology·2020
Same author

Effluent lipopolysaccharide is a prompt marker of peritoneal dialysis-related gram-negative peritonitis.

Peritoneal dialysis international : journal of the International Society for Peritoneal Dialysis·2020
Same author

Current Status and Prospects in the Treatment of Erectile Dysfunction by Adipose-Derived Stem Cells in the Diabetic Animal Model.

Sexual medicine reviews·2020
Same author

Low-Complexity Adaptive Signal Detection for Mobile Molecular Communication.

IEEE transactions on nanobioscience·2020
Same author

Corrigendum to "HMGB1 contributes to adriamycin-induced cardiotoxicity via up-regulating autophagy" [Toxicol. Lett. 292 (2018) 115-121].

Toxicology letters·2019
Same author

Programming nanoparticle valence bonds with single-stranded DNA encoders.

Nature materials·2019
Same journal

Silicon-Mediated Laser Shock Synthesis of Nanocrystalline Diamonds from Low-Rank Coal.

ACS nano·2026
Same journal

Precursor-Engineered Strategy for Constructing Supported Tetra-Atom Pt Clusters to Boost Propane Dehydrogenation under Direct Resistive Heating.

ACS nano·2026
Same journal

Enterohepatic Circulation of Polystyrene Nanoplastics Promotes Intestinal Inflammation by Impairing Enteric Neurons.

ACS nano·2026
Same journal

Triboelectric Spectroscopy for Identification of Metal Ion Valence States in Aqueous Solutions.

ACS nano·2026
Same journal

Beyond the Continuum Theory: Conductance Scaling and Correlated Imaging in Atom-Scale Artificial Ion Channels.

ACS nano·2026
Same journal

Selenium-Induced Directional Growth of Ultrathin Nanowires with Subnano Amorphous Shells for High-Performance Multifunctional Electrocatalysis.

ACS nano·2026
See all related articles

Related Experiment Video

Updated: Apr 30, 2026

Chemical Dimerization-Induced Protein Condensates on Telomeres
08:52

Chemical Dimerization-Induced Protein Condensates on Telomeres

Published on: April 12, 2021

2.5K

Kinetics of DNA tile dimerization.

Shuoxing Jiang1, Hao Yan, Yan Liu

  • 1Department of Chemistry and Biochemistry and Biodesign Institute, Arizona State University , Tempe, Arizona 85287, United States.

ACS Nano
|May 6, 2014
PubMed
Summary
This summary is machine-generated.

Understanding DNA nanoarchitectures is key for designing efficient molecular systems. This study reveals how DNA tile orientation, flexibility, and sticky ends influence dimerization rates, guiding improved self-assembly and molecular robotics.

More Related Videos

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

1.2K
Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques
05:58

Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques

Published on: September 6, 2024

1.6K

Related Experiment Videos

Last Updated: Apr 30, 2026

Chemical Dimerization-Induced Protein Condensates on Telomeres
08:52

Chemical Dimerization-Induced Protein Condensates on Telomeres

Published on: April 12, 2021

2.5K
Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

1.2K
Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques
05:58

Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques

Published on: September 6, 2024

1.6K

Area of Science:

  • Biochemistry
  • Nanotechnology
  • Molecular Biology

Background:

  • Understanding molecular interactions within DNA nanoarchitectures is crucial for predicting their physical behavior.
  • Designing complex higher-order structures requires insight into spatial and temporal component interactions.

Purpose of the Study:

  • To investigate spatial factors influencing the kinetics of bivalent, double-helical (DH) tile dimerization.
  • To elucidate how orientation, sticky end characteristics, flexibility, and tile size affect dimerization rates.

Main Methods:

  • Kinetic analysis of bivalent double-helical (DH) tile dimerization.
  • Examination of spatial factors including sticky end (SE) orientation and number, and DH domain flexibility.
  • Application of the Arrhenius equation to analyze dimerization rate constants.

Main Results:

  • Increased nucleation opportunities and aligned SEs accelerate DH tile dimerization.
  • Increased tile flexibility leads to slower dimerization rates, which can be mitigated by restricting flexibility.
  • More rigid tiles exhibit higher dimerization rates due to a dominant pre-exponential factor in the Arrhenius equation.

Conclusions:

  • Spatial factors significantly impact DNA tile dimerization kinetics.
  • Findings provide guidance for designing efficient DNA tile-based self-assembly and molecular robotics.
  • Optimizing tile design can improve yield and efficiency in nucleic acid systems.