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Related Experiment Videos

Changes in concanavalin A-reactive proteins in inflammatory disorders.

B Silvestrini1, A Guglielmotti, L Saso

  • 1Institute of Pharmacology and Pharmacognosy, University of Rome, Italy.

Clinical Chemistry
|November 1, 1989
PubMed
Summary

Concanavalin A (Con A) reactivity increased in serum proteins during inflammation in rats and in patients with rheumatoid arthritis and systemic lupus erythematosus. This suggests altered protein glycosylation is linked to autoimmune diseases.

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Area of Science:

  • Biochemistry
  • Immunology
  • Glycobiology

Background:

  • Inflammation and autoimmune diseases are characterized by complex molecular changes.
  • Concanavalin A (Con A) is a lectin that binds to specific carbohydrate structures on glycoproteins, making it useful for studying protein glycosylation.
  • Changes in protein glycosylation patterns can serve as biomarkers for disease states.

Purpose of the Study:

  • To investigate quantitative changes in Concanavalin A (Con A)-reactive proteins in serum during inflammation.
  • To assess the diagnostic potential of Con A-reactive proteins in inflammatory and autoimmune diseases.
  • To identify specific glycoproteins whose Con A-reactivity is altered in disease.

Main Methods:

  • Induction of inflammation in rats using fermented yeast.

Related Experiment Videos

  • Collection and analysis of serum samples from rats and patients with inflammatory/autoimmune diseases.
  • Lectin blotting using Con A to detect Con A-reactive proteins.
  • Densitometric scanning for quantitative analysis of specific glycoproteins (alpha 2-macroglobulin, P-150, P-95, P-40, P-35).

    • Main Results:

    • Induced inflammation in rats led to a time-dependent increase in Con A-reactivity of serum proteins.
    • Elevated Con A-reactivity was observed in patients with rheumatoid arthritis and systemic lupus erythematosus compared to healthy donors.
    • Specific proteins, including alpha 2-macroglobulin, showed increased Con A-reactivity during inflammation and in autoimmune conditions.

    Conclusions:

    • Con A-reactive proteins in serum can serve as indicators of inflammation and autoimmune disease.
    • Increased Con A-reactivity of alpha 2-macroglobulin, a non-acute-phase protein, suggests altered glycosylation in autoimmune diseases.
    • Further study of Con A-reactive proteins may enhance understanding of autoimmune disease etiology and pathophysiology.