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Protein Dynamics in Living Cells

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Updated: Apr 30, 2026

TIRFM and pH-sensitive GFP-probes to Evaluate Neurotransmitter Vesicle Dynamics in SH-SY5Y Neuroblastoma Cells: Cell Imaging and Data Analysis

TIRFM and pH-sensitive GFP-probes to Evaluate Neurotransmitter Vesicle Dynamics in SH-SY5Y Neuroblastoma Cells: Cell Imaging and Data Analysis

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Acidic-pH-activatable fluorescence probes for visualizing exocytosis dynamics.

Daisuke Asanuma¹, Yousuke Takaoka, Shigeyuki Namiki

¹Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo (Japan).

Angewandte Chemie (International Ed. in English)

|May 8, 2014

Summary

Researchers developed new fluorescent probes to visualize cellular secretion processes like exocytosis and endocytosis. This technique offers precise spatiotemporal tracking of vesicle dynamics, aiding the study of cellular pH changes.

Keywords:

acidity exocytosis fluorescent probes spatiotemporal dynamics vesicular imaging

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Area of Science:

Cellular Biology
Biochemistry
Biophysics

Background:

Understanding exocytosis dynamics is vital for studying cellular secretion.
Current live imaging methods for exocytosis have significant limitations in precision and reliability.

Purpose of the Study:

To develop and validate novel small-molecular fluorescent probes for live imaging of exocytosis.
To enable sensitive and reliable visualization of vesicular dynamics and pH changes during secretion.

Main Methods:

Design and synthesis of small-molecular fluorescent probes.
Chemical optimization of probes for sensing acidic intravesicular pH.
Application of probes for live imaging of cellular exocytosis and endocytosis.

Main Results:

Successfully synthesized fluorescent probes capable of sensing acidic intravesicular pH.
Demonstrated sensitive and reliable visualization of vesicular dynamics post-stimulation.
Achieved high spatiotemporal precision in imaging exocytosis, endocytosis, and reacidification.

Conclusions:

The developed fluorescent probes provide a straightforward and powerful tool for studying dynamic cellular processes.
This technique enhances the spatiotemporal understanding of exocytosis and related phenomena involving pH changes.
Expected to advance research in cellular secretion and vesicle trafficking.