Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

2.9K
The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
2.9K
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

7.7K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
7.7K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.9K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.9K
Mass Analyzers: Overview01:13

Mass Analyzers: Overview

2.1K
The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...
2.1K
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

2.1K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
2.1K
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

3.1K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
3.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enhancing the Sensitivity of Mass Spectrometry Imaging through Spatial Signal Averaging.

Journal of the American Society for Mass Spectrometry·2026
Same author

Top-Down Mass Spectrometry of a Clinical Antibody Light Chain Using the Omnitrap-Orbitrap-Booster Platform.

Journal of the American Society for Mass Spectrometry·2025
Same author

Top-Down and Middle-Down Mass Spectrometry of Antibodies.

Molecular & cellular proteomics : MCP·2025
Same author

High-Performance Data Acquisition for Fourier Transform Mass Spectrometry.

Chimia·2025
Same author

Deciphering Isotopic Fine Structures of Silylated Compounds in Gas Chromatography-Vacuum Photoionization Orbitrap Mass Spectrometry of Bio-Oils.

Journal of the American Society for Mass Spectrometry·2024
Same author

Revisiting Dissolved Organic Matter Analysis Using High-Resolution Trapped Ion Mobility and FT-ICR Mass Spectrometry.

Journal of the American Society for Mass Spectrometry·2024

Related Experiment Video

Updated: Apr 12, 2026

Analyzing Large Protein Complexes by Structural Mass Spectrometry
15:35

Analyzing Large Protein Complexes by Structural Mass Spectrometry

Published on: June 19, 2010

25.0K

From high- to super-resolution mass spectrometry.

Yury O Tsybin1

  • 1Biomolecular Mass Spectrometry Laboratory, Ecole Polytechnique Fédérale de Lausanne, EPFL BCH 4307, CH-1015 Lausanne, Switzerland.

Chimia
|May 8, 2014
PubMed
Summary

High-resolution mass spectrometry (MS) advances structural analysis of complex biological and environmental samples. A new super-resolution MS technique overcomes speed and resolution limitations for broader scientific applications.

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Environmental Science

Background:

  • High-resolution mass spectrometry (MS) is crucial for analyzing complex biological and environmental samples.
  • Fourier transform mass spectrometry (FTMS) is a leading high-resolution MS technique.

Purpose of the Study:

  • To summarize laboratory developments in FTMS for structural analysis.
  • To introduce super-resolution mass spectrometry (SRMS) to overcome current limitations.

Main Methods:

  • Top-down, middle-down, and extended bottom-up mass spectrometry for protein analysis.
  • Extended bottom-up proteomics for complex mixtures.
  • Analysis of crude oil fractions.
  • Development of super-resolution mass spectrometry (SRMS).

More Related Videos

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
10:01

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

Published on: November 28, 2017

20.6K
Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry
07:33

Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry

Published on: October 15, 2018

15.2K

Related Experiment Videos

Last Updated: Apr 12, 2026

Analyzing Large Protein Complexes by Structural Mass Spectrometry
15:35

Analyzing Large Protein Complexes by Structural Mass Spectrometry

Published on: June 19, 2010

25.0K
Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
10:01

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

Published on: November 28, 2017

20.6K
Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry
07:33

Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry

Published on: October 15, 2018

15.2K

Main Results:

  • Demonstrated success in structural analysis of large proteins, proteomes, and complex mixtures.
  • Identified a trade-off between MS acquisition speed and resolution.
  • Pioneered SRMS to enhance FTMS capabilities.

Conclusions:

  • FTMS has significantly advanced molecular structural analysis.
  • Current MS techniques face limitations in speed and resolution for complex applications.
  • SRMS offers a path forward for enhanced MS applications in life, environmental, and material sciences.