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Identification of specific corrinoids reveals corrinoid modification in dechlorinating microbial communities
Yujie Men1, Erica C Seth2, Shan Yi1
1Department of Civil and Environmental Engineering, University of California, Berkeley, CA, 94720, USA.
Environmental Microbiology
|May 8, 2014
Summary
Microbial communities exchange essential corrinoids for survival. This study shows Dehalococcoides mccartyi remodels imported p-cresolylcobamide and DMB to produce cobalamin for dechlorination.
Area of Science:
- Microbiology
- Biochemistry
- Environmental Science
Background:
- Corrinoids are vital cofactors for many organisms, but microbial production and exchange are poorly understood.
- Microbes often rely on external sources for corrinoids, especially those with specific enzyme requirements like Dehalococcoides mccartyi.
Purpose of the Study:
- To investigate corrinoid profiles, lower ligand bases, and microbial associations in communities containing Dehalococcoides mccartyi.
- To understand corrinoid exchange and modification mechanisms crucial for microbial survival and function.
Main Methods:
- Utilized a novel liquid chromatography tandem mass spectrometry (LC-MS/MS) method for corrinoid detection.
- Analyzed microbial communities with an obligate requirement for benzimidazole-containing corrinoids for trichloroethene respiration.
Main Results:
- p-Cresolylcobamide ([p-Cre]Cba) and cobalamin were the most abundant corrinoids.
- Veillonellaceae family members were linked to [p-Cre]Cba production.
- Observed correlation between [p-Cre]Cba decrease, cobalamin increase, and D. mccartyi growth during dechlorination.
Conclusions:
- Dehalococcoides mccartyi likely remodels imported [p-Cre]Cba and 5,6-dimethylbenzimidazole (DMB) into cobalamin for its cofactor needs.
- DMB plays a significant role in corrinoid remodeling within these communities.
- Findings offer insights into microbial corrinoid dynamics and potential applications in chlorinated solvent bioremediation.
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