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Semple rabies vaccine: presence of myelin basic protein and proteolipid protein and its activity in experimental
R S Javier1, T Kunishita, F Koike
1Division of Demyelinating Disease and Aging, National Institute of Neuroscience, NCNP, Tokyo, Japan.
Abstract:
Myelin basic protein (MBP) as a cause of postvaccinal encephalomyelitis (PVE) due to Semple rabies vaccine (SRV) has been suggested in previous reports. No actual measurement of MBP in SRV was done. In this study we detected MBP and PLP in the vaccine using immunological methods. The vaccine was found to contain 28 micrograms MBP per ml vaccine. Inoculation with SRV plus adjuvant resulted in the development of experimental allergic encephalomyelitis (EAE) in 2 of 3 guinea pigs. For control, chick embryo vaccine (CEV) was used and MBP was not detected. EAE was not induced in animals inoculated with it. These results suggest that MBP in vaccines may play a decisive role in the production of PVE.
Insights
Myelin basic protein (MBP) in Semple rabies vaccine (SRV) may cause postvaccinal encephalomyelitis (PVE). This study detected MBP in SRV, and inoculation induced experimental allergic encephalomyelitis (EAE) in guinea pigs.
Area of Science:
- Neuroimmunology
- Vaccinology
Background:
- Previous reports suggested myelin basic protein (MBP) as a cause of postvaccinal encephalomyelitis (PVE) from Semple rabies vaccine (SRV).
- No direct measurement of MBP in SRV had been performed.
Purpose of the Study:
- To detect and quantify MBP in SRV.
- To investigate the potential of SRV to induce experimental allergic encephalomyelitis (EAE).
Main Methods:
- Immunological methods were used to detect MBP and proteolipid protein (PLP) in SRV.
- Guinea pigs were inoculated with SRV plus adjuvant, and a control group received chick embryo vaccine (CEV).
Main Results:
- SRV contained 28 micrograms of MBP per ml.
- Inoculation with SRV plus adjuvant induced EAE in 2 out of 3 guinea pigs.
- CEV, which did not contain detectable MBP, did not induce EAE.
Conclusions:
- MBP is present in SRV.
- MBP in SRV may play a critical role in the development of PVE.
