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Development of a new rhodamine-based FRET platform and its application as a Cu2+ probe
Xiaoyu Guan1, Weiying Lin, Weimin Huang
1State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, P. R. China. weiyinglin2013@163.com.
Abstract:
We have constructed a new rhodamine-based FRET platform, which was then used to develop a ratiometric fluorescent Cu(2+) probe. The novel Cu(2+) probe exhibits several favorable features including a large variation in the emission ratio, well-resolved emission peaks, high sensitivity, and high selectivity. Importantly, it is suitable for fluorescence imaging in living cells.

