Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Splicing01:32

RNA Splicing

53.3K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
53.3K
RNA Splicing01:32

RNA Splicing

15.9K
15.9K
Alternative RNA Splicing02:18

Alternative RNA Splicing

20.5K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
20.5K
Alternative RNA Splicing02:18

Alternative RNA Splicing

4.2K
4.2K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

6.6K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
6.6K
Pre-mRNA Processing: RNA Splicing01:36

Pre-mRNA Processing: RNA Splicing

5.6K
5.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spatiomolecular mapping reveals anatomical organization of heterogeneous cell types in the human nucleus accumbens.

Neuron·2026
Same author

Long QT Syndrome Type 5 With Coexisting KCNE1 and RYR2 Variants: A Diagnostic Ambiguity.

Clinical case reports·2026
Same author

A scalable approach to investigating sequence-to-function predictions from personal genomes.

Nature methods·2026
Same author

KLF4 Initiates Dedifferentiation of Systemic Sclerosis Lung Fibroblasts.

Cells·2026
Same author

Sex Disparity in Systemic Sclerosis-Associated Pulmonary Fibrosis.

International journal of molecular sciences·2026
Same author

Multi-Omic, Multi-Tissue Responses to Acute Exercise in Sedentary Adults: Findings from the Molecular Transducers of Physical Activity Consortium.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Apr 30, 2026

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

5.9K

Transcriptome analysis reveals differential splicing events in IPF lung tissue.

Tracy Nance, Kevin S Smith, Vanessa Anaya

    Plos One
    |May 9, 2014
    PubMed
    Summary

    Idiopathic pulmonary fibrosis (IPF) involves disrupted gene expression and alternative splicing. RNA sequencing revealed significant differences in IPF lung tissue, highlighting potential new therapeutic targets for this complex disease.

    More Related Videos

    Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts
    11:19

    Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts

    Published on: October 9, 2016

    16.1K
    Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
    11:48

    Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

    Published on: October 9, 2014

    12.5K

    Related Experiment Videos

    Last Updated: Apr 30, 2026

    Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
    08:35

    Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

    Published on: June 24, 2021

    5.9K
    Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts
    11:19

    Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts

    Published on: October 9, 2016

    16.1K
    Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
    11:48

    Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

    Published on: October 9, 2014

    12.5K

    Area of Science:

    • Genomics
    • Molecular Biology
    • Pulmonary Medicine

    Background:

    • Idiopathic pulmonary fibrosis (IPF) is a complex lung disease characterized by disrupted protein networks.
    • Traditional methods like microarrays have limitations in detecting alternative splicing events.

    Purpose of the Study:

    • To investigate differential gene expression and alternative splicing in IPF lung tissue using RNA sequencing.
    • To identify genes and splicing events potentially involved in IPF pathogenesis.

    Main Methods:

    • RNA sequencing (RNA-Seq) of messenger RNA from 8 IPF lung samples and 7 healthy controls.
    • Analysis of differential gene expression and differential splicing events.
    • Quantitative PCR (qPCR) validation of differential exon usage for COL6A3 and POSTN genes.

    Main Results:

    • Identified 873 differentially expressed genes (FDR<5%) and 440 unique genes with significant differential splicing events (FDR<5%) in IPF.
    • Validated differential exon usage in COL6A3 and POSTN, genes encoding extracellular matrix proteins.
    • Observed increased gene-level expression of periostin, previously associated with IPF progression.

    Conclusions:

    • Alternative splicing of genes like COL6A3 and POSTN may play a role in IPF pathogenesis.
    • RNA-Seq provides valuable insights into gene regulation in IPF, complementing existing data.
    • An interactive web application was developed to explore RNA-Seq and microarray data for IPF research.