Related Experiment Video

Updated: Aug 14, 2026

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
08:09

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Published on: March 24, 2017

Serological mapping of HLA-epitopes with monoclonal antibodies and its interpretation by sequenced HLA-molecules

J Harpprecht1, K Olde, E Westphal

  • 1Department of Immunology, University of Kiel, FRG.

Tissue Antigens
|September 1, 1989
PubMed

For the study of the epitope distribution on HLA class I molecules, blocking studies were carried out with a competitive radioimmuno assay using 3H labelled monoclonal antibodies (MAbs) against various HLA epitopes. The results were discussed with the help of known HLA amino acid sequences, and the positions of the following epitopes were suggested: A2/Aw69; A2/28; A2/11/25/26/28/29/30/31/Aw33/34; A25/32; Bw4; B7/Bw22,42; Bw6.

More Related Videos

Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation08:07

Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation

Published on: September 6, 2017

Overlapping Peptide Library to Map Qa-1 Epitopes in a Protein08:04

Overlapping Peptide Library to Map Qa-1 Epitopes in a Protein

Published on: December 20, 2017

Related Concept Videos

Hybridoma Technology01:31

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...