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Updated: Apr 30, 2026

Establishment of a Human Multiple Myeloma Xenograft Model in the Chicken to Study Tumor Growth, Invasion and Angiogenesis
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Augmented expression of urokinase plasminogen activator and extracellular matrix proteins associates with multiple

Rehan Khan1, Nidhi Gupta, Raman Kumar

  • 1Department of Biochemistry, All India Institute of Medical Sciences (AIIMS), New Delhi, 110029, India.

Clinical & Experimental Metastasis
|May 9, 2014
PubMed

Insights

This study found increased levels of extracellular matrix (ECM) proteins and urokinase plasminogen activator (uPA) in multiple myeloma (MM) patients. These markers correlate with disease severity and may indicate active MM.

Area of Science:

  • Oncology
  • Biochemistry
  • Molecular Biology

Background:

  • Multiple myeloma (MM) is a B cell malignancy involving malignant plasma cell proliferation.
  • Tumor cell and extracellular matrix (ECM) interactions are crucial for MM invasion and metastasis.

Purpose of the Study:

  • To investigate the levels of ECM proteins (laminin, nidogen, fibulin 1) and urokinase plasminogen activator (uPA) in MM patients.
  • To analyze the correlation of these molecules with disease severity and their potential as biomarkers.

Main Methods:

  • ELISA and quantitative PCR were used to measure protein and mRNA levels of uPA and ECM proteins in 80 subjects.
  • Pearson correlation analysis was performed to assess interrelationships and correlation with disease severity.

Main Results:

  • Significantly elevated serum and mRNA levels of ECM proteins (laminin, nidogen, fibulin 1) and uPA were observed in MM patients compared to healthy controls.
  • A significant positive correlation was found between all measured ECM proteins and uPA.
  • The levels of these molecules correlated with the severity of the disease.

Conclusions:

  • Elevated circulatory and mRNA levels of ECM proteins and uPA suggest their role in MM progression.
  • These molecules may serve as potential biomarkers for active multiple myeloma.
  • The strong association between ECM proteins and uPA indicates a potential therapeutic target for MM growth and spread.

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