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DNA vaccination by intradermal electroporation induces long-lasting immune responses in rhesus macaques
Viraj Kulkarni1, Margherita Rosati, Rashmi Jalah
1Human Retrovirus Pathogenesis Section, Vaccine Branch, Center for Cancer Research, National Cancer Institute, Frederick, MD, USA.
Background:
A desirable HIV vaccine should induce protective long-lasting humoral and cellular immune responses.
Methods:
Macaques were immunized by env DNA, selected from a panel of recently transmitted SIVmac251 Env using intradermal electroporation as vaccine delivery method and magnitude, breadth and longevity of humoral and cellular immune responses.
Results:
The macaques developed high, long-lasting humoral immune responses with neutralizing capacity against homologous and heterologous Env. The avidity of the antibody responses was also preserved over 1-year follow-up. Analysis of cellular immune responses demonstrated induction of Env-specific memory T cells harboring granzyme B, albeit their overall levels were low. Similar to the humoral responses, the cellular immunity was persistent over the ~1-year follow-up.
Conclusion:
These data show that vaccination by this intradermal DNA delivery regimen is able to induce potent and durable immune responses in macaques.
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