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Inhibitory effects of α-pinene on hepatoma carcinoma cell proliferation
Wei-Qiang Chen1, Bin Xu, Jian-Wen Mao
1School of Basic Medicine and Guangdong Key Laboratory for Bioactive Drug Research, Guangdong Pharmaceutical University, Guangzhou, ChinaE-mail : cwq2187@163.com.
Background:
Pine needle oil from crude extract of pine needles has anti-tumor effects, but the effective component is not known.
Methods:
In the present study, compounds from a steam distillation extract of pine needles were isolated and characterized. Alpha-pinene was identified as an active anti-proliferative compound on hepatoma carcinoma BEL-7402 cells using the MTT assay.
Results:
Further experiments showed that α-pinene inhibited BEL-7402 cells by arresting cell growth in the G2/M phase of the cell cycle, downregulating Cdc25C mRNA and protein expression, and reducing cycle dependence on kinase 1(CDK1) activity.
Conclusion:
Taken together, these findings indicate that α-pinene may be useful as a potential anti-tumor drug.
