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qPCR for quantification of transgene expression and determination of transgene copy number
1Chemistry and Molecular Biosciences, The School of Biological Sciences, University of Queensland, St Lucia Campus, Brisbane, QLD, 4072, Australia, s.fletcher@uq.edu.au.
Abstract:
Quantitative real-time PCR (qPCR) is a mature technology that can be used to accurately quantify the number of copies of a target nucleic acid in a sample. Here, we describe a method for using this technology to determine the copy number of a transgene stably integrated into a plant's genome and to ascertain the level of transgene expression.
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