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    Optimizing suction pressure in a novel tissue suction method enhances liver transfection efficiency for naked DNA (pDNA) in mice. A 5 kPa pressure demonstrated effectiveness with minimal hepatic toxicity, improving gene delivery safety and accuracy.

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    Area of Science:

    • Biotechnology
    • Molecular Biology
    • Gene Delivery

    Background:

    • A novel in vivo tissue suction method was previously developed for delivering naked nucleic acids.
    • The impact of suction pressure parameters on transfection efficiency and safety remained uninvestigated.

    Purpose of the Study:

    • To develop a computer-controlled system for precise suction pressure regulation.
    • To investigate the effects of suction pressure magnitude, waveform, and duration on liver transfection efficiency of plasmid DNA (pDNA) in mice.

    Main Methods:

    • A computer system was engineered to control suction pressure during transfection.
    • Mice livers were transfected with naked pDNA encoding luciferase under varying suction conditions.
    • Luciferase expression levels and hepatic toxicity were assessed.

    Main Results:

    • The liver suction method at 5 kPa proved effective and exhibited the lowest hepatic toxicity.
    • Suction pressure waveform significantly influenced luciferase expression levels.
    • A single suction period was sufficient for successful transfection.

    Conclusions:

    • The developed computer system enables accurate and safe application of the tissue suction method.
    • Optimized suction pressure conditions, specifically 5 kPa, enhance gene delivery efficiency while minimizing liver toxicity.
    • This method offers a promising approach for in vivo gene transfection in the liver.