Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Microbial Morphologies

Microbial Morphologies

Bacterial and archaeal cells exhibit remarkable diversity in shape and structure, critical in their adaptability and functionality. Among bacteria, the most commonly observed shapes include cocci and bacilli. Cocci are spherical and may exist singly or in groupings such as pairs (diplococci), chains (streptococci), clusters (staphylococci), or tetrads. Bacilli, in contrast, are rod-shaped and can also occur as single cells, in pairs, or chains, depending on their environmental and genetic...

Studying the Cytoskeleton

Studying the Cytoskeleton

The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

MiR-1 mediates autophagy via ATG14 in sheep Leydig cells infected with <i>Brucella melitensis</i> strain BA0711.

Frontiers in veterinary science·2026

Same author

Social Partner Effects on Type 2 Diabetes Prevention, Management, and Spillover Health Outcomes: Single-Arm Pre-Post Pilot Intervention.

JMIR formative research·2026

Same author

Exploring Preferences and Priorities in Advanced Parkinson's Disease: A Discrete Choice Experiment.

Neurology and therapy·2026

Same author

Microbiota-accessible carbohydrates enhance gut microbiota stability and antibiotic resilience through production of quorum sensing molecule AI-2.

Gut microbes reports·2026

Same author

Glycaemic Outcomes of a Novel Model-Predictive-Control-Based Hybrid Closed-Loop System in Chinese Adolescents and Adults With Type 1 Diabetes: A Multicentre Pivotal Trial.

Diabetes, obesity & metabolism·2026

Same author

Research Progress on Common Sports Injuries Among Youth Ice Hockey Players and Prevention Strategies: A Narrative Review.

Sports (Basel, Switzerland)·2025

Same journal

Isolation of Mesenchymal Stem Cell-Derived Extracellular Vesicles.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Modeling Melanoma Immune Surveillance by CAR-T Cells in Human Skin Organoids.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Stepwise Optimization of a Matrigel-Based In Vitro Angiogenesis Assay for Reproducible and Quantifiable 2D-Tube Formation Using HUVECs.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Quantifying Mechanical Properties of Fresh Ovarian Tissue with Optical Brillouin Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

3D Chromatin Architecture During Early Development: New Methods and New Findings.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Metabolic Plasticity in Embryogenesis Throughout the Lens of NAD<sup></sup>.

Methods in molecular biology (Clifton, N.J.)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Apr 30, 2026

Quantitative Approaches for Studying Cellular Structures and Organelle Morphology in Caenorhabditis elegans

Quantitative Approaches for Studying Cellular Structures and Organelle Morphology in Caenorhabditis elegans

Published on: July 5, 2019

Microscopic analysis: morphotypes and cellular appendages.

Olga Zaborina¹, John Alverdy, Megha Shah

¹Laboratory for Surgical Infection Research and Therapeutics, Department of Surgery, University of Chicago, 5841 S. Maryland MC5032, room J557B, Chicago, IL, 60637, USA, ozaborin@surgery.bsd.uchicago.edu.

Methods in Molecular Biology (Clifton, N.J.)

|May 14, 2014

Summary

This summary is machine-generated.

Pseudomonas aeruginosa produces specific cellular appendages under phosphate-limited conditions. These appendages correlate with increased adhesion and virulence in multidrug-resistant strains.

More Related Videos

Preparation of Prokaryotic and Eukaryotic Organisms Using Chemical Drying for Morphological Analysis in Scanning Electron Microscopy SEM

Preparation of Prokaryotic and Eukaryotic Organisms Using Chemical Drying for Morphological Analysis in Scanning Electron Microscopy SEM

Published on: January 7, 2019

Morphological Analysis of Drosophila Larval Peripheral Sensory Neuron Dendrites and Axons Using Genetic Mosaics

Morphological Analysis of Drosophila Larval Peripheral Sensory Neuron Dendrites and Axons Using Genetic Mosaics

Published on: November 7, 2011

Related Experiment Videos

Last Updated: Apr 30, 2026

Quantitative Approaches for Studying Cellular Structures and Organelle Morphology in Caenorhabditis elegans

Quantitative Approaches for Studying Cellular Structures and Organelle Morphology in Caenorhabditis elegans

Published on: July 5, 2019

Preparation of Prokaryotic and Eukaryotic Organisms Using Chemical Drying for Morphological Analysis in Scanning Electron Microscopy SEM

Preparation of Prokaryotic and Eukaryotic Organisms Using Chemical Drying for Morphological Analysis in Scanning Electron Microscopy SEM

Published on: January 7, 2019

Morphological Analysis of Drosophila Larval Peripheral Sensory Neuron Dendrites and Axons Using Genetic Mosaics

Morphological Analysis of Drosophila Larval Peripheral Sensory Neuron Dendrites and Axons Using Genetic Mosaics

Published on: November 7, 2011

Area of Science:

Microbiology
Microbial Pathogenesis
Cell Biology

Background:

Microscopic analysis is crucial for characterizing microbial morphotypes.
Transmission electron microscopy (TEM) and immunoelectron microscopy (IEM) are standard techniques for visualizing microbial cells and protein localization.
Certain Pseudomonas aeruginosa strains exhibit unique cellular appendages under specific environmental conditions.

Purpose of the Study:

To describe a technique for detecting type-specific cellular appendages in Pseudomonas aeruginosa.
To investigate the relationship between appendage production and bacterial phenotype.
To highlight the importance of appendage visualization for understanding their structure and function.

Main Methods:

Utilized microscopic analysis, including TEM and IEM, for visualization.
Cultured Pseudomonas aeruginosa strains under varying phosphate conditions (rich vs. depleted media).
Correlated the presence or absence of appendages with bacterial phenotypes.

Main Results:

Described a technique for detecting type-specific cellular appendages in Pseudomonas aeruginosa.
Observed that appendage production is suppressed in phosphate-rich media and enhanced in phosphate-depleted media.
Found a correlation between the presence/absence of appendages and adhesive/virulent phenotypes in multidrug-resistant strains.

Conclusions:

The described technique enables the visualization of type-specific cellular appendages in Pseudomonas aeruginosa.
Appendage production is regulated by phosphate availability and linked to bacterial virulence.
Further research into appendage structure and function is warranted.