Membrane proteomics of Pseudomonas aeruginosa

In recent years gel-free proteomics approaches have been increasingly used for global quantitative proteome analyses of multiple prokaryotic organisms, including Pseudomonas aeruginosa. A major advantage of this method is its suitability for the investigation of membrane proteomes. In this chapter, we present a protocol for preparation of proteins from the inner and outer membrane of P. aeruginosa PAO1 grown as a biofilm culture. Parameters for quantitative protein measurements by 2D-LC-MS/MS are described.