Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Chromatin Immunoprecipitation- ChIP

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...

Immunoprecipitation

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Multicenter Genomic Analysis of Carbapenem-Resistant <i>Pseudomonas aeruginosa</i> in Austrian Community Hospitals Reveals Limited Carbapenemase Prevalence and Absence of Interhospital Clonal Spread.

Antibiotics (Basel, Switzerland)·2026

Same author

The role of whole genome sequencing in antimicrobial susceptibility prediction of bacteria: 2025 update from the European Committee on Antimicrobial Susceptibility Testing Subcommittee.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2026

Same author

Primary extragonadal germ cell tumours: Real-world data from a high-volume centre.

BJUI compass·2026

Same author

Nanomotion-enabled ultra-rapid antibiotic susceptibility testing with magnetic bead-based pathogen enrichment for accelerated sepsis diagnostics.

Scientific reports·2026

Same author

Pseudopaline-mediated zinc uptake by <i>Pseudomonas aeruginosa</i> drives clinically relevant phenotypes and infection outcomes.

Infection and immunity·2026

Same author

A distinct monocyte transcriptional state links systemic immune dysregulation to pulmonary impairment in long COVID.

Nature immunology·2026

Search research articles

Related Experiment Video

Updated: Apr 30, 2026

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing ChIP-seq

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing ChIP-seq

Published on: April 19, 2013

Chromatin immunoprecipitation for ChIP-chip and ChIP-seq.

Sebastian Schulz¹, Susanne Häussler

¹Twincore, Centre of Experimental and Clinical Infection Research, Feodor-Lynen-Str. 7, 30625, Hannover, Germany.

Methods in Molecular Biology (Clifton, N.J.)

|May 14, 2014

Summary

Understanding bacterial adaptation requires studying gene regulation. Chromatin immunoprecipitation (ChIP) methods like ChIP-chip and ChIP-seq map protein-DNA interactions, revealing transcriptional regulator roles in gene expression and bacterial environmental adaptation.

More Related Videos

Chromatin Immunoprecipitation ChIP Protocol for Low-abundance Embryonic Samples

Chromatin Immunoprecipitation ChIP Protocol for Low-abundance Embryonic Samples

Published on: August 29, 2017

TChIP-Seq: Cell-Type-Specific Epigenome Profiling

TChIP-Seq: Cell-Type-Specific Epigenome Profiling

Published on: January 23, 2019

Related Experiment Videos

Last Updated: Apr 30, 2026

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing ChIP-seq

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing ChIP-seq

Published on: April 19, 2013

Chromatin Immunoprecipitation ChIP Protocol for Low-abundance Embryonic Samples

Chromatin Immunoprecipitation ChIP Protocol for Low-abundance Embryonic Samples

Published on: August 29, 2017

TChIP-Seq: Cell-Type-Specific Epigenome Profiling

TChIP-Seq: Cell-Type-Specific Epigenome Profiling

Published on: January 23, 2019

Area of Science:

Microbiology and Molecular Biology
Genomics and Bioinformatics

Background:

Bacterial adaptation to environmental changes relies on intricate gene regulatory mechanisms.
Identifying the precise roles of individual transcriptional regulators is crucial for understanding these processes.

Purpose of the Study:

To investigate how single transcriptional regulators modulate bacterial gene expression.
To map genome-wide protein-DNA interactions for transcription factors.

Main Methods:

Utilizing chromatin immunoprecipitation (ChIP) techniques, specifically ChIP-chip and ChIP-seq.
Generating genome-wide maps of protein-DNA interactions to identify transcription factor regulons.

Main Results:

ChIP-chip and ChIP-seq successfully generated genome-wide maps of protein-DNA interactions.
These methods identified primary regulons for specific transcription factors.

Conclusions:

ChIP-chip and ChIP-seq are powerful tools for mapping transcription factor binding sites.
Integrating this data with transcriptome analyses enables the construction of bacterial regulatory networks, advancing our understanding of adaptation.