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Targeted DNA Methylation Analysis by Next-generation Sequencing
Published on: February 24, 2015
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Qualitative and quantitative detection of methylation at CpG sites using the fluorescein-dGTP incorporated asymmetric
Tingting Hong1, Tianlu Wang, Zhiyong He
1College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei 430072, P. R. China. xzhou@whu.edu.cn.
Abstract:
The methylation status of each CpG site can be monitored by Fl-dGTP incorporated asymmetric PCR assay. The ability of quantitative detection makes it a good choice for detecting partial methylation at CpG sites compared with others. And the monitoring is not limited to sites within PCR primers or restriction enzyme-recognition sites.

