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Updated: Apr 29, 2026
MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
Published on: October 7, 2025
Graphene fluorescence switch-based cooperative amplification: a sensitive and accurate method to detection microRNA
1College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Normal University , Jinan, 250014 Shandong, People's Republic of China.
We developed a sensitive method for detecting microRNAs (miRNAs) using graphene oxide and DNA amplification. This technique enhances fluorescence signals for accurate cancer biomarker detection and clinical diagnosis.
Area of Science:
- Biochemistry
- Molecular Biology
- Nanotechnology
Background:
- MicroRNAs (miRNAs) are crucial regulators of biological processes and are recognized as biomarkers and therapeutic targets in cancer.
- Sensitive and accurate detection of miRNAs is essential for understanding their roles in cancer and for clinical diagnosis.
Purpose of the Study:
- To develop a stable, sensitive, and specific method for detecting microRNAs (miRNAs).
- To leverage cooperative amplification with graphene oxide (GO) and multimolecule labeling for enhanced miRNA detection.
Main Methods:
- Utilized graphene oxide (GO) to adsorb and protect target miRNAs from enzymatic degradation.
- Employed a fluorescence switch-based circular exponential amplification strategy involving hairpin probes and nicking enzymes.
- Enhanced fluorescence signals through multimolecule labeling of double-stranded DNA (dsDNA) with SYBR Green I (SG).
Main Results:
- Achieved remarkable signal amplification by converting small amounts of target miRNA into numerous DNA triggers.
- Demonstrated enhanced detection sensitivity due to multimolecule binding of SG to dsDNA and low fluorescence background from GO.
- Successfully developed a sensitive and accurate method for miRNA detection with a high signal-to-noise ratio.
Conclusions:
- The proposed method offers a novel approach for reliable miRNA quantification.
- This strategy holds promise for advancing medical research and early clinical diagnostics of cancer.
