Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

RACE - Rapid Amplification of cDNA Ends

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...

Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Prediction of disease resilience of pigs using multi-omics data.

Journal of animal science and biotechnology·2026

Same author

Genetic analysis of High Immune Response technology and relationships with disease resilience in pigs.

Journal of animal science·2026

Same author

Responses to the backtest as genetic indicators of disease resilience in grow-finish pigs.

Journal of animal science·2026

Same author

Genetics of retroactive measures of stress response in pigs before and after exposure to a disease challenge.

G3 (Bethesda, Md.)·2026

Same author

Daily feed intake patterns of purebred nucleus boars as genetic indicators for disease resilience of crossbred barrows under a natural polymicrobial disease challenge.

Journal of animal science·2025

Same author

Early Identification of Individual Nursery Pigs at Risk of Requiring Health Treatment Using Machine Learning.

Journal of animal science·2025

Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Apr 29, 2026

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Published on: August 3, 2018

RNA amplification for pseudogene detection using RNA-seq.

Stephen C M Tsoi¹, Michael K Dyck

¹Agricultural Genomics and Proteomics Unit, Core Research Laboratory, Department of Agricultural, Food and Nutritional Science, 4-32G Agriculture/Forestry Centre, University of Alberta, Edmonton, AB, Canada, T6G 2P5, scmtsoi@gmail.com.

Methods in Molecular Biology (Clifton, N.J.)

|May 15, 2014

Summary

This summary is machine-generated.

A new, rapid RNA amplification and cDNA synthesis method enables transcriptomic analysis from just 10 ng of total RNA from porcine embryos. This technique supports RNA-sequencing for detecting protein-coding and noncoding RNAs.

More Related Videos

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Published on: March 10, 2020

Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA

Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA

Published on: January 22, 2018

Related Experiment Videos

Last Updated: Apr 29, 2026

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Published on: August 3, 2018

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Published on: March 10, 2020

Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA

Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA

Published on: January 22, 2018

Area of Science:

Molecular Biology
Genomics
Developmental Biology

Background:

Advancements in cDNA synthesis methods allow transcriptomic analysis from minimal biological samples.
RNA-sequencing (RNA-seq) is a powerful tool for studying gene expression.
Porcine embryos represent a valuable model for developmental studies but yield small sample sizes.

Purpose of the Study:

To develop a rapid and efficient method for RNA amplification and double-stranded cDNA synthesis.
To enable comprehensive transcriptomic profiling of porcine embryos using minimal RNA input.
To validate the suitability of the synthesized cDNA for RNA-sequencing.

Main Methods:

Extraction of high-quality total RNA from porcine embryos.
A streamlined protocol for RNA amplification and double-stranded cDNA synthesis.
RNA-sequencing library preparation and analysis.

Main Results:

Successful amplification and synthesis of double-stranded cDNA from as little as 10 ng of total RNA.
The generated cDNA is suitable for sensitive RNA-sequencing.
The method allows for the detection of both protein-coding transcripts and noncoding RNAs, including pseudogenes.

Conclusions:

This rapid RNA amplification and cDNA synthesis method significantly lowers the RNA input requirement for transcriptomic studies.
It provides a valuable tool for researchers working with limited biological samples, such as embryos.
The technique facilitates comprehensive RNA-seq analysis, enhancing our understanding of gene expression in developmental processes.