Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The Cellular Response Capacity as Diagnostic Head Start in Neutrophil Endotoxemia Sensing.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026

Same author

Fluorescence Correlation Spectroscopy to Examine Protein-Lipid Interactions in Membranes.

Methods in molecular biology (Clifton, N.J.)·2026

Same author

Ion- and Temperature-Programmable Reconfiguration of Subcompartments in Synthetic Cells.

Chembiochem : a European journal of chemical biology·2026

Same author

The cellular response capacity (CRC) as a novel immunomonitoring approach in sepsis.

Military Medical Research·2026

Same author

Optimizing spatial organization of FtsZ rings for large-scale constriction in synthetic cells.

Nature communications·2026

Same author

On the nature of the earliest known lifeforms.

eLife·2026

Same journal

conMItion: an R package adjusting confounding factors for associations in multi-omics.

Bioinformatics (Oxford, England)·2026

Same journal

SpaMFG: a Spatial Multi-omics Integration Method based on Feature Grouping.

Bioinformatics (Oxford, England)·2026

Same journal

CSCN: Inference of Cell-Specific Causal Networks Using Single-Cell RNA-Seq Data.

Bioinformatics (Oxford, England)·2026

Same journal

Sparse CCA-Based Mediation Analysis with High-Dimensional Exposures and Mediators.

Bioinformatics (Oxford, England)·2026

Same journal

Enhancing Cross-Context Generalization in Drug Perturbation Prediction with a Multimodal Conditional Diffusion Framework.

Bioinformatics (Oxford, England)·2026

Same journal

Primer Design through Submodular Function Estimation.

Bioinformatics (Oxford, England)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Apr 29, 2026

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

Published on: December 1, 2018

PyCorrFit-generic data evaluation for fluorescence correlation spectroscopy.

Paul Müller¹, Petra Schwille¹, Thomas Weidemann¹

¹Biotechnology Center, Technische Universität Dresden, 01307 Dresden and Max Planck Institute of Biochemistry, Cellular and Molecular Biophysics, 82152 Martinsried, Germany.

Bioinformatics (Oxford, England)

|May 15, 2014

Summary

This summary is machine-generated.

PyCorrFit is a new graphical user interface for analyzing fluorescence correlation spectroscopy (FCS) data. This software simplifies the fitting of experimental results to theoretical models, aiding researchers in data evaluation.

More Related Videos

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Published on: December 11, 2021

Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy

Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy

Published on: April 25, 2021

Related Experiment Videos

Last Updated: Apr 29, 2026

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

Published on: December 1, 2018

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Published on: December 11, 2021

Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy

Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy

Published on: April 25, 2021

Area of Science:

Biophysics
Physical Chemistry
Spectroscopy

Background:

Fluorescence Correlation Spectroscopy (FCS) is a powerful technique for studying molecular dynamics.
Analyzing FCS data often involves fitting experimental results to theoretical models, which can be complex.
Existing tools may lack comprehensive features or user-friendliness for specialized FCS data evaluation.

Purpose of the Study:

To introduce PyCorrFit, a novel graphical user interface (GUI) designed for FCS data analysis.
To provide researchers with a user-friendly platform for fitting experimental FCS data to theoretical models.
To offer a specialized set of tools for efficient and accurate FCS data evaluation.

Main Methods:

Development of a Python-based graphical user interface (GUI).
Implementation of algorithms for fitting theoretical model functions to experimental data.
Support for multiple data file formats commonly used in FCS experiments.
Integration of specialized tools for FCS data evaluation.

Main Results:

PyCorrFit offers a versatile platform for analyzing FCS data.
The software supports a wide range of data file formats.
Includes specialized tools to enhance the accuracy and efficiency of FCS data fitting and evaluation.
Provides a user-friendly interface for complex data analysis.

Conclusions:

PyCorrFit is a valuable and accessible tool for researchers using fluorescence correlation spectroscopy.
The software simplifies the process of model fitting and data evaluation in FCS.
Freely available source code and pre-compiled binaries promote widespread adoption and use.