[A photometric method for assessing the proliferation of cell cultures]

A possibility to evaluate the quantity of cultured cells was examined using the optical density of the stain eluate after absorbtion by cells in the course of staining of the cellular monolayer. The investigation of the growth dynamics of primary trypsinized cultures of embryonic fibroblasts using the proposed criteria has shown its relationship with the quantity of cells in culture. It is proposed that this method may be used for quantitative characteristics of cell cultures.