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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: Apr 29, 2026

Combining Fluidic Devices with Microscopy and Flow Cytometry to Study Microbial Transport in Porous Media Across Spatial Scales

Combining Fluidic Devices with Microscopy and Flow Cytometry to Study Microbial Transport in Porous Media Across Spatial Scales

Published on: November 25, 2020

A flow cytometry-based submicron-sized bacterial detection system using a movable virtual wall.

Hyoungseon Choi¹, Chang Su Jeon, Inseong Hwang

¹Interdisciplinary Program in Bioengineering, Seoul National University, 28 Yongon-dong, Chongno-gu, Seoul, Korea.

|May 16, 2014

Summary

This summary is machine-generated.

This study introduces a novel flow cytometry system for detecting submicron bacteria using a virtual liquid wall. This adaptable method allows for precise control of channel dimensions, enabling sensitive detection of pathogens like Francisella tularensis.

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Area of Science:

Microfluidics
Biosensing
Pathogen Detection

Background:

Accurate detection of pathogenic bacteria is crucial for public health.
Existing microfluidic impedance sensors face challenges in fabricating narrow channels for submicron bacteria.
Varied bacterial sizes necessitate adaptable detection systems.

Purpose of the Study:

To develop a flow cytometry-based system for detecting submicron-sized pathogenic bacteria.
To demonstrate the ability to adjust microfluidic channel dimensions using a virtual liquid wall.
To simultaneously monitor impedance and fluorescence for bacterial discrimination.

Main Methods:

Utilized a flow cytometry setup with a movable virtual wall composed of a non-conducting fluid.
Adjusted microfluidic channel dimensions by varying flow rates of sample and virtual wall fluids.
Detected submicron-sized Francisella tularensis and discriminated bacterial mixtures (F. tularensis and E. coli BL21) using DC impedance and fluorescence.

Main Results:

Successfully controlled microfluidic channel width using a virtual liquid wall.
Achieved sensitive detection of submicron-sized Francisella tularensis.
Demonstrated simultaneous impedance and fluorescence monitoring for differentiating bacterial mixtures based on size.

Conclusions:

The proposed flow cytometry system offers a promising approach for detecting submicron-sized pathogenic microbes.
The virtual liquid wall technology provides an adaptable and cost-effective solution for microfluidic bacterial sensing.
This system enhances capabilities for identifying and discriminating various bacterial species.