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Updated: Apr 29, 2026

In Vivo Calcium Imaging of Lateral-line Hair Cells in Larval Zebrafish
Published on: November 28, 2018
ATP hydrolysis is critically required for function of CaV1.3 channels in cochlear inner hair cells via fueling Ca2+
Simon Weiler1, Stefanie Krinner2, Aaron B Wong3
1InnerEarLab, Department of Otolaryngology, University Medical Center Göttingen, 37099 Göttingen, Germany, International Max-Planck Research School Neuroscience, Göttingen Graduate School for Neuroscience and Molecular Biosciences, and.
Abstract:
Sound encoding is mediated by Ca(2+) influx-evoked release of glutamate at the ribbon synapse of inner hair cells. Here we studied the role of ATP in this process focusing on Ca(2+) current through CaV1.3 channels and Ca(2+) homeostasis in mouse inner hair cells. Patch-clamp recordings and Ca(2+) imaging demonstrate that hydrolyzable ATP is essential to maintain synaptic Ca(2+) influx in inner hair cells via fueling Ca(2+)-ATPases to avoid an increase in cytosolic [Ca(2+)] and subsequent Ca(2+)/calmodulin-dependent inactivation of CaV1.3 channels.
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