Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Exploring the somatic mutational landscape of ovarian cancer in Estonia.

Acta oncologica (Stockholm, Sweden)·2026

Same author

Exploring the hereditary genetic mutational landscape of breast and ovarian cancer in Estonia.

Scientific reports·2026

Same author

Shell Engineering of ITO Nanocrystals via Seed-Mediated Growth and Precursor Crowding for Broadband Visible- to-Infrared Absorption.

Chemistry of materials : a publication of the American Chemical Society·2025

Same author

Enhancing the Performance and Reliability of an Automotive Reed Sensor Through Spring Integration and Advanced Manufacturing.

Sensors (Basel, Switzerland)·2025

Same author

Nanoscale Copper-Tin Dioxide Interfaces for Efficient CO<sub>2</sub> Electroreduction to Formic Acid and Formate at High Rates.

ChemSusChem·2025

Same author

A Printable OECT for Simple Integration in Nitrocellulose-Based Assays.

ACS sensors·2025

Same journal

A pump-free gravity-driven microfluidic chip for rapid RPA-LFS-based detection of Magnaporthe oryzae AvrPi9 gene.

Biomedical microdevices·2026

Same journal

Mechanotherapeutic biomaterials: Overcoming physical barriers to enhance intratumoral drug delivery in solid tumours.

Biomedical microdevices·2026

Same journal

Reversibly-sealable microfluidic platform for multi-molecule gradient delivery to large adherent cell cultures.

Biomedical microdevices·2026

Same journal

3D printed chip as platform to vascularize hiPSCs-derived kidney organoids.

Biomedical microdevices·2026

Same journal

Ingestible smart capsules: from engineering innovation to GI drug delivery.

Biomedical microdevices·2026

Same journal

An inexpensive, portable, refrigeration-free, ready-to-use microfluidic device for real-time multiplexed molecular detection of HIV, HBV, and HCV.

Biomedical microdevices·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Apr 29, 2026

Polymer Microarrays for High Throughput Discovery of Biomaterials

Polymer Microarrays for High Throughput Discovery of Biomaterials

Published on: January 25, 2012

A polymer lab-on-a-chip for genetic analysis using the arrayed primer extension on microarray chips.

Simone L Marasso¹, Domenico Mombello, Matteo Cocuzza

¹Xlab - Materials and Microsystems Laboratory, Applied Science and Technology Department (DISAT), Polytechnic of Turin - Latemar Unit, Via Lungo Piazza d'Armi 6, 10034, Chivasso (Turin), Italy, simone.marasso@polito.it.

Biomedical Microdevices

|May 17, 2014

Summary

This summary is machine-generated.

This study developed a polymer lab-on-a-chip (LOC) for detecting Single Nucleotide Polymorphisms (SNPs) using the Arrayed Primer EXtension (APEX) protocol. The LOC achieved comparable results to conventional methods with reduced reagent volumes, demonstrating its potential for genetic disorder testing.

More Related Videos

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

Related Experiment Videos

Last Updated: Apr 29, 2026

Polymer Microarrays for High Throughput Discovery of Biomaterials

Polymer Microarrays for High Throughput Discovery of Biomaterials

Published on: January 25, 2012

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

Area of Science:

Biotechnology
Genetics
Microfluidics

Background:

Single Nucleotide Polymorphisms (SNPs) are crucial genetic markers for various disorders.
Hereditary hearing loss is a significant condition with clear genetic underpinnings, driving the need for efficient diagnostic tools.
Conventional genetic analysis methods can be time-consuming and require large reagent volumes.

Purpose of the Study:

To develop and validate a polymer lab-on-a-chip (LOC) device for efficient Single Nucleotide Polymorphisms (SNPs) detection.
To implement the Arrayed Primer EXtension (APEX) genetic protocol on the LOC for analyzing SNPs related to hereditary hearing loss.
To assess the performance of the LOC-based APEX assay compared to traditional methods.

Main Methods:

Fabrication of a two-level polymer lab-on-a-chip (LOC) using Poly (methyl methacrylate) (PMMA) via MEMS technology.
Integration of the LOC with low-density (10 mutations) and high-density (245 mutations) DNA microarray chips.
Implementation of the Arrayed Primer EXtension (APEX) genetic protocol on the LOC for SNP analysis.

Main Results:

The LOC-based APEX assay demonstrated signal-to-noise ratios and efficiency comparable to conventional protocols.
Successful detection of SNPs associated with hereditary hearing loss using both low and high-density microarray chips.
A significant reduction in Polymerase Chain Reaction (PCR) volume from 30 μL to 10 μL was achieved with the low-density chip.

Conclusions:

The developed polymer lab-on-a-chip (LOC) is a viable platform for executing the APEX genetic protocol for SNP detection.
The LOC offers a miniaturized and efficient approach for genetic analysis, comparable in performance to standard methods.
This technology holds promise for accessible and cost-effective genetic testing, particularly for hereditary disorders like hearing loss.