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An Aptamer-based Sensor for Unchelated GadoliniumIII
Published on: January 9, 2017
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An extremely sensitive aptasensor based on interfacial energy transfer between QDS SAMs and GO
Xiangying Sun1, Bin Liu1, Chuanxiao Yang1
1College of Materials Science and Engineering, Huaqiao University, 361021 Xiamen, China.
Summary
This study introduces a novel biosensing platform using fluorescent multilayers and graphene oxide for highly sensitive detection of thrombin and DNA. The method achieves excellent sensitivity, with detection limits in the picomolar and femtomolar ranges.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Analytical Chemistry
Background:
- Biosensing requires highly sensitive and selective detection methods for biomolecules like thrombin and DNA.
- Graphene oxide (GO) offers excellent fluorescence quenching properties valuable for sensing applications.
- Self-assembled multilayers provide a versatile platform for immobilizing recognition elements.
Purpose of the Study:
- To design and develop a novel fluorescent self-assembled multilayer biosensor.
- To utilize interfacial fluorescence resonance energy transfer (FRET) for sensitive biomolecule detection.
- To establish a new interfacial sensing method for thrombin and DNA with high sensitivity.
Main Methods:
- Fabrication of fluorescent self-assembled multilayers with immobilized thrombin aptamers and single-stranded DNA (ssDNA).
- Utilizing graphene oxide as a fluorescence quencher for interfacial FRET.
- Characterization of the multilayer structure and its sensing performance.
Main Results:
- The developed multilayer system effectively sensed biomolecules via interfacial FRET to graphene oxide.
- High fluorescence quenching efficiency of GO and the self-assembled membrane contributed to good biosensing sensitivity.
- Achieved a detection limit of 16.2 pM for thrombin and 72.6 fM for DNA.
Conclusions:
- A highly sensitive interfacial sensing method for thrombin and DNA detection was successfully established.
- The fluorescent multilayer-graphene oxide platform demonstrates significant potential for advanced biosensing applications.
- The method offers a promising approach for sensitive and selective detection of specific DNA sequences and proteins.
