A rational approach for generating cardiac troponin I selective Spiegelmers
Zsuzsanna Szeitner1, Gergely Lautner, Szilvia K Nagy
1Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Semmelweis University, Tűzoltó u. 37-47, H-1094 Budapest, Hungary. meszaros.tamas@med.semmelweis-univ.hu.
Chemical Communications (Cambridge, England)
|May 20, 2014
Abstract:
We report the first protein selective Spiegelmers of diagnostic relevance by rational identification of a target epitope and reverse screening of Spiegelmer candidates following the selection procedure. Application of the presented approach resulted in isolation of cardiac troponin I selective Spiegelmers with low nanomolar dissociation constant and functionality in serum.
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