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Loop-mediated Isothermal Amplification LAMP Assays for the Species-specific Detection of Eimeria that Infect Chickens
Published on: February 20, 2015
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PCR Assay Specific for Chicken Feces.
Cindy R Cisar1, Tatsuya Akiyama1, Jonathan Hatley1
1Department of Natural Sciences, 600 N. Grand Avenue, Northeastern State University, Tahlequah, OK, 74464.
Proceedings of the Oklahoma Academy of Science
|May 20, 2014
Summary
Researchers developed a new PCR assay to detect chicken feces. This method uses specific primers to identify chicken Bacteroidales DNA, aiding in tracking fecal contamination sources.
Area of Science:
- Microbiology
- Environmental Science
- Molecular Biology
Background:
- Bacteroidales are common fecal anaerobic bacteria in warm-blooded animals.
- Some Bacteroidales strains exhibit host specificity, suggesting potential as indicators.
Purpose of the Study:
- To investigate Bacteroidales strains from chicken feces for their utility as indicators of chicken fecal contamination.
- To develop a specific molecular tool for detecting chicken fecal sources.
Main Methods:
- Amplification, cloning, and sequencing of Bacteroidales 16S rRNA genes from chicken feces.
- Phylogenetic analysis comparing chicken sequences with human and bovine Bacteroidales.
- Design and testing of specific primers using PCR assays for chicken fecal DNA detection.
Main Results:
- One primer set (chBact F1 and chBact R16) specifically amplified chicken fecal DNA.
- The assay did not amplify DNA from wild turkey, cat, bovine, or deer feces.
- Chicken fecal DNA in straw-based litter was detected, but not in wood shavings-based litter.
Conclusions:
- A novel PCR assay using specific Bacteroidales primers can accurately detect chicken fecal contamination.
- This assay is a potential tool for identifying chicken feces and tracing contamination sources in watersheds.
